| RRC ID |
84029
|
| Author |
Chen X, Wang K, Mufti FUD, Xu D, Zhu C, Huang X, Zeng C, Jin Q, Huang X, Yan YH, Dong MQ, Feng X, Shi Y, Kennedy S, Guang S.
|
| Title |
Germ granule compartments coordinate specialized small RNA production.
|
| Journal |
Nat Commun
|
| Abstract |
Germ granules are biomolecular condensates present in most animal germ cells. One function of germ granules is to help maintain germ cell totipotency by organizing mRNA regulatory machinery, including small RNA-based gene regulatory pathways. The C. elegans germ granule is compartmentalized into multiple subcompartments whose biological functions are largely unknown. Here, we identify an uncharted subcompartment of the C. elegans germ granule, which we term the E granule. The E granule is nonrandomly positioned within the germ granule. We identify five proteins that localize to the E granule, including the RNA-dependent RNA polymerase (RdRP) EGO-1, the Dicer-related helicase DRH-3, the Tudor domain-containing protein EKL-1, and two intrinsically disordered proteins, EGC-1 and ELLI-1. Localization of EGO-1 to the E granule enables synthesis of a specialized class of 22G RNAs, which derive exclusively from 5' regions of a subset of germline-expressed mRNAs. Defects in E granule assembly elicit disordered production of endogenous siRNAs, which disturbs fertility and the RNAi response. Our results define a distinct subcompartment of the C. elegans germ granule and suggest that one function of germ granule compartmentalization is to facilitate the localized production of specialized classes of small regulatory RNAs.
|
| Volume |
15(1)
|
| Pages |
5799
|
| Published |
2024-7-10
|
| DOI |
10.1038/s41467-024-50027-3
|
| PII |
10.1038/s41467-024-50027-3
|
| PMID |
38987544
|
| PMC |
PMC11236994
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Cytoplasmic Granules* / metabolism
DEAD-box RNA Helicases / genetics
DEAD-box RNA Helicases / metabolism
Germ Cells* / metabolism
Intrinsically Disordered Proteins / genetics
Intrinsically Disordered Proteins / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA-Dependent RNA Polymerase / genetics
RNA-Dependent RNA Polymerase / metabolism
|
| Resource |
| C.elegans |
tm1296
tm892
tm894
tm3169
tm2645
tm3315
tm2223
tm1724
tm1357
tm2428
tm1019
tm1195
tm1753
tm1144
tm887
tm1283
tm914 |
