論文 - 詳細
| RRC ID | 84029 |
|---|---|
| 著者 | Chen X, Wang K, Mufti FUD, Xu D, Zhu C, Huang X, Zeng C, Jin Q, Huang X, Yan YH, Dong MQ, Feng X, Shi Y, Kennedy S, Guang S. |
| タイトル | Germ granule compartments coordinate specialized small RNA production. |
| ジャーナル | Nat Commun |
| Abstract |
Germ granules are biomolecular condensates present in most animal germ cells. One function of germ granules is to help maintain germ cell totipotency by organizing mRNA regulatory machinery, including small RNA-based gene regulatory pathways. The C. elegans germ granule is compartmentalized into multiple subcompartments whose biological functions are largely unknown. Here, we identify an uncharted subcompartment of the C. elegans germ granule, which we term the E granule. The E granule is nonrandomly positioned within the germ granule. We identify five proteins that localize to the E granule, including the RNA-dependent RNA polymerase (RdRP) EGO-1, the Dicer-related helicase DRH-3, the Tudor domain-containing protein EKL-1, and two intrinsically disordered proteins, EGC-1 and ELLI-1. Localization of EGO-1 to the E granule enables synthesis of a specialized class of 22G RNAs, which derive exclusively from 5' regions of a subset of germline-expressed mRNAs. Defects in E granule assembly elicit disordered production of endogenous siRNAs, which disturbs fertility and the RNAi response. Our results define a distinct subcompartment of the C. elegans germ granule and suggest that one function of germ granule compartmentalization is to facilitate the localized production of specialized classes of small regulatory RNAs. |
| 巻・号 | 15(1) |
| ページ | 5799 |
| 公開日 | 2024-7-10 |
| DOI | 10.1038/s41467-024-50027-3 |
| PII | 10.1038/s41467-024-50027-3 |
| PMID | 38987544 |
| PMC | PMC11236994 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cytoplasmic Granules* / metabolism DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism Germ Cells* / metabolism Intrinsically Disordered Proteins / genetics Intrinsically Disordered Proteins / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism RNA-Dependent RNA Polymerase / genetics RNA-Dependent RNA Polymerase / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1296 tm892 tm894 tm3169 tm2645 tm3315 tm2223 tm1724 tm1357 tm2428 tm1019 tm1195 tm1753 tm1144 tm887 tm1283 tm914 |