Reference - Detail
| RRC ID | 84037 |
|---|---|
| Author | Eliad B, Schneider N, Ben-Naim Zgayer O, Amichan Y, Glaser F, Erdmann EA, Rajendren S, Hundley HA, Lamm AT. |
| Title | ADBP-1 regulates ADR-2 nuclear localization to control editing substrate selection. |
| Journal | Nucleic Acids Res |
| Abstract |
Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is a prevalent and conserved RNA modification. While A-to-I RNA editing is essential in mammals, in Caenorhabditis elegans, it is not, making them invaluable for RNA editing research. In C. elegans, ADR-2 is the sole catalytic A-to-I editing enzyme, and ADR-1 is an RNA editing regulator. ADAR localization is well-studied in humans but not well-established in C. elegans. In this study, we examine the cellular and tissue-specific localization of ADR-2. We show that while ADR-2 is present in most cells in the embryo, at later developmental stages, its expression is both tissue- and cell-type-specific. Additionally, both ADARs are mainly in the nucleus. ADR-2 is adjacent to the chromosomes during the cell cycle. We show that the nuclear localization of endogenous ADR-2 depends on ADBP-1, not ADR-1. In adbp-1 mutant worms, ADR-2 is mislocalized, while ADR-1 is not, leading to decreased editing levels and de-novo editing, mostly in exons, suggesting that ADR-2 is also functional in the cytoplasm. Besides, mutated ADBP-1 affects gene expression. Furthermore, we show that ADR-2 targets adenosines with different surrounding nucleotides in exons and introns. Our findings indicate that ADR-2 cellular localization is highly regulated and affects its function. |
| Volume | 52(16) |
| Pages | 9501-9518 |
| Published | 2024-9-9 |
| DOI | 10.1093/nar/gkae641 |
| PII | 7717836 |
| PMID | 39036970 |
| PMC | PMC11381337 |
| MeSH | Adenosine Deaminase* / genetics Adenosine Deaminase* / metabolism Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Nucleus* / genetics Cell Nucleus* / metabolism Mutation RNA Editing* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Substrate Specificity |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm668 |