Reference - Detail
| RRC ID | 84074 |
|---|---|
| Author | Sandhu A, Lyu X, Wan X, Meng X, Tang NH, Gonzalez G, Syed IN, Chen L, Jin Y, Chisholm AD. |
| Title | The microtubule regulator EFA-6 forms cortical foci dependent on its intrinsically disordered region and interactions with tubulins. |
| Journal | Cell Rep |
| Abstract |
The EFA6 protein family, originally identified as Sec7 guanine nucleotide exchange factors, has also been found to regulate cortical microtubule (MT) dynamics. Here, we find that in the mature C. elegans epidermal epithelium, EFA-6 forms punctate foci in specific regions of the apical cortex, dependent on its intrinsically disordered region (IDR). The EFA-6 IDR can form biomolecular condensates in vitro. In genetic screens for mutants with altered GFP::EFA-6 localization, we identified a gain-of-function (gf) mutation in α-tubulin tba-1 that induces ectopic EFA-6 foci in multiple cell types. Lethality of tba-1(gf) is partially suppressed by loss of function in efa-6. The ability of TBA-1(gf) to trigger ectopic EFA-6 foci requires β-tubulin TBB-2 and the chaperon EVL-20/Arl2. tba-1(gf)-induced EFA-6 foci display slower turnover, contain the MT-associated protein TAC-1/TACC, and require the EFA-6 MT elimination domain (MTED). Our results reveal functionally important crosstalk between cellular tubulins and cortical MT regulators in vivo. |
| Volume | 43(10) |
| Pages | 114776 |
| Published | 2024-10-22 |
| DOI | 10.1016/j.celrep.2024.114776 |
| PII | S2211-1247(24)01127-6 |
| PMID | 39305484 |
| PMC | PMC11972086 |
| MeSH | Animals Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Guanine Nucleotide Exchange Factors* / chemistry Guanine Nucleotide Exchange Factors* / genetics Guanine Nucleotide Exchange Factors* / metabolism Intrinsically Disordered Proteins / chemistry Intrinsically Disordered Proteins / metabolism Microtubules* / metabolism Mutation Protein Binding Tubulin* / metabolism |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm3124 tmC27 |