| RRC ID |
84074
|
| Author |
Sandhu A, Lyu X, Wan X, Meng X, Tang NH, Gonzalez G, Syed IN, Chen L, Jin Y, Chisholm AD.
|
| Title |
The microtubule regulator EFA-6 forms cortical foci dependent on its intrinsically disordered region and interactions with tubulins.
|
| Journal |
Cell Rep
|
| Abstract |
The EFA6 protein family, originally identified as Sec7 guanine nucleotide exchange factors, has also been found to regulate cortical microtubule (MT) dynamics. Here, we find that in the mature C. elegans epidermal epithelium, EFA-6 forms punctate foci in specific regions of the apical cortex, dependent on its intrinsically disordered region (IDR). The EFA-6 IDR can form biomolecular condensates in vitro. In genetic screens for mutants with altered GFP::EFA-6 localization, we identified a gain-of-function (gf) mutation in α-tubulin tba-1 that induces ectopic EFA-6 foci in multiple cell types. Lethality of tba-1(gf) is partially suppressed by loss of function in efa-6. The ability of TBA-1(gf) to trigger ectopic EFA-6 foci requires β-tubulin TBB-2 and the chaperon EVL-20/Arl2. tba-1(gf)-induced EFA-6 foci display slower turnover, contain the MT-associated protein TAC-1/TACC, and require the EFA-6 MT elimination domain (MTED). Our results reveal functionally important crosstalk between cellular tubulins and cortical MT regulators in vivo.
|
| Volume |
43(10)
|
| Pages |
114776
|
| Published |
2024-10-22
|
| DOI |
10.1016/j.celrep.2024.114776
|
| PII |
S2211-1247(24)01127-6
|
| PMID |
39305484
|
| PMC |
PMC11972086
|
| MeSH |
Animals
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Guanine Nucleotide Exchange Factors* / chemistry
Guanine Nucleotide Exchange Factors* / genetics
Guanine Nucleotide Exchange Factors* / metabolism
Intrinsically Disordered Proteins / chemistry
Intrinsically Disordered Proteins / metabolism
Microtubules* / metabolism
Mutation
Protein Binding
Tubulin* / metabolism
|
| Resource |
| C.elegans |
tm3124
tmC27 |
