論文 - 詳細
| RRC ID | 84131 |
|---|---|
| 著者 | Morrow CS, Yao P, Vergani-Junior CA, Anekal PV, Montero Llopis P, Miller JW, Benayoun BA, Mair WB. |
| タイトル | Endogenous mitochondrial NAD(P)H fluorescence can predict lifespan. |
| ジャーナル | Commun Biol |
| Abstract |
Many aging clocks have recently been developed to predict health outcomes and deconvolve heterogeneity in aging. However, existing clocks are limited by technical constraints, such as low spatial resolution, long processing time, sample destruction, and a bias towards specific aging phenotypes. Therefore, here we present a non-destructive, label-free and subcellular resolution approach for quantifying aging through optically resolving age-dependent changes to the biophysical properties of NAD(P)H in mitochondria through fluorescence lifetime imaging (FLIM) of endogenous NAD(P)H fluorescence. We uncover age-dependent changes to mitochondrial NAD(P)H across tissues in C. elegans that are associated with a decline in physiological function and construct non-destructive, label-free and cellular resolution models for prediction of age, which we refer to as "mito-NAD(P)H age clocks." Mito-NAD(P)H age clocks can resolve heterogeneity in the rate of aging across individuals and predict remaining lifespan. Moreover, we spatiotemporally resolve age-dependent changes to mitochondria across and within tissues, revealing multiple modes of asynchrony in aging and show that longevity is associated with a ubiquitous attenuation of these changes. Our data present a high-resolution view of mitochondrial NAD(P)H across aging, providing insights that broaden our understanding of how mitochondria change during aging and approaches which expand the toolkit to quantify aging. |
| 巻・号 | 7(1) |
| ページ | 1551 |
| 公開日 | 2024-11-21 |
| DOI | 10.1038/s42003-024-07243-w |
| PII | 10.1038/s42003-024-07243-w |
| PMID | 39572679 |
| PMC | PMC11582643 |
| MeSH | Aging Animals Caenorhabditis elegans* / metabolism Caenorhabditis elegans* / physiology Fluorescence Longevity* Mitochondria* / metabolism NAD / metabolism NADP* / metabolism Optical Imaging / methods |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 43 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tmC5 |