論文 - 詳細
| RRC ID | 84186 |
|---|---|
| 著者 | Wang Y, Chow CH, Zhang Y, Huang M, Higazy R, Ramakrishnan N, Chen L, Chen X, Deng Y, Wang S, Zhang C, Ma C, Sugita S, Gao S. |
| タイトル | The exocytosis regulator complexin controls spontaneous synaptic vesicle release in a CAPS-dependent manner at C. elegans excitatory synapses. |
| ジャーナル | PLoS Biol |
| Abstract |
The balance between synaptic excitation and inhibition (E/I) is essential for coordinating motor behavior, yet the differential roles of exocytosis regulators in this balance are less understood. In this study, we investigated the roles of 2 conserved exocytosis regulators, complexin/CPX-1 and CAPS/UNC-31, in excitatory versus inhibitory synapses at Caenorhabditis elegans neuromuscular junctions. cpx-1 null mutants exhibited a marked increase in spontaneous release specifically at excitatory synapses, alongside an unequal reduction in excitatory and inhibitory evoked release. A clamping-specific knockin mutant, cpx-1(Δ12), which preserved evoked release, also showed a biased enhancement in excitatory spontaneous release. Conversely, the unc-31 null mutation, while maintaining normal spontaneous release, displayed a more pronounced reduction in evoked release at excitatory synapses. Notably, we found that CPX-1's clamping function is dependent on UNC-31 and is sensitive to external Ca2+. Pull-down experiments confirmed that CAPS/UNC-31 does not directly interact with complexin, implying an indirect regulatory mechanism. Moreover, complexin regulates activity-dependent synaptic plasticity, which is also UNC-31 dependent. The unexpected role of CAPS/UNC-31 in the absence of CPX-1 clamping function may underpin the synaptic E/I balance and coordinated behavioral outputs in different species. |
| 巻・号 | 23(2) |
| ページ | e3003023 |
| 公開日 | 2025-2-1 |
| DOI | 10.1371/journal.pbio.3003023 |
| PII | PBIOLOGY-D-24-02914 |
| PMID | 39913617 |
| PMC | PMC11838871 |
| MeSH | Adaptor Proteins, Vesicular Transport* / genetics Adaptor Proteins, Vesicular Transport* / metabolism Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans* / physiology Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Calcium / metabolism Calcium-Binding Proteins* / genetics Calcium-Binding Proteins* / metabolism Exocytosis* / physiology Mutation Nerve Tissue Proteins* / genetics Nerve Tissue Proteins* / metabolism Neuromuscular Junction / metabolism Neuromuscular Junction / physiology Neuronal Plasticity Synapses* / metabolism Synaptic Transmission / physiology Synaptic Vesicles* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1377 tm1104 |