論文 - 詳細
| RRC ID | 84240 |
|---|---|
| 著者 | Ujie Y, Saito S, Fukaya K, Urabe D, Yaguchi T, Arai MA. |
| タイトル | Aspergillus terreus IFM 65899-THP-1 cells interaction triggers production of the natural product butyrolactone Ia, an immune suppressive compound. |
| ジャーナル | Sci Rep |
| Abstract |
We focused on the possibility that pathogenic microorganisms might produce immune suppressors to evade the action of immune cells. Based on this possibility, we have recently developed new co-culture method of pathogenic actinomyces and immune cells, however, the interaction mechanism between pathogens and cells was still unclear. In this report, co-culturing pathogenic fungi and immune cells were investigated. Pathogenic fungus Aspergillus terreus IFM 65899 and THP-1 cells were co-cultured and isolated a co-culture specific compound, butyrolactone Ia (1). 1 inhibits the production of nitric oxide by RAW264 cells and exhibits regulatory effects on autophagy, suggesting 1 plays a defensive role in the response of A. terreus IFM 65899 to immune cells. Furthermore, dialysis experiments and micrographs indicated that "physical interaction" between A. terreus IFM 65899 and THP-1 cells may be required for the production of 1. This is the first report of co-culture method of fungi with immune cells and its interaction mechanism. |
| 巻・号 | 14(1) |
| ページ | 28278 |
| 公開日 | 2024-11-16 |
| DOI | 10.1038/s41598-024-79837-7 |
| PII | 10.1038/s41598-024-79837-7 |
| PMID | 39550448 |
| PMC | PMC11569209 |
| MeSH | 4-Butyrolactone* / analogs & derivatives 4-Butyrolactone* / metabolism Animals Aspergillus* / metabolism Autophagy Biological Products / pharmacology Coculture Techniques* Humans Mice Nitric Oxide* / metabolism RAW 264.7 Cells THP-1 Cells |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原真核微生物 | Aspergillus terreus IFM 65899 |
| ヒト・動物細胞 | RAW 264(RCB0535) |