論文 - 詳細
| RRC ID | 84404 |
|---|---|
| 著者 | Iikawa H, Nishina A, Morita M, Atsuta Y, Hayashi Y, Saito D. |
| タイトル | Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin. |
| ジャーナル | Dev Growth Differ |
| Abstract |
Avian species are essential resources for human society, with their preservation and utilization heavily dependent on primordial germ cells (PGCs). However, efficient methods for isolating live PGCs from embryos remain elusive in avian species beyond chickens, and even in chickens, existing techniques have shown limited efficiency. In this study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, using Lycopersicon Esculentum (Tomato) lectin (LEL). Notably, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative and migratory potential of chicken PGCs. This approach is anticipated to significantly contribute to the conservation, research, and agricultural industries related to avian species globally. |
| 巻・号 | 66(9) |
| ページ | 452-461 |
| 公開日 | 2024-12-1 |
| DOI | 10.1111/dgd.12948 |
| PMID | 39520225 |
| PMC | PMC11659090 |
| MeSH | Animals Birds / embryology Birds / metabolism Chickens Germ Cells* / cytology Germ Cells* / metabolism Plant Lectins / metabolism |
| IF | 1.723 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ニワトリ・ウズラ | BL‐E strain of brawn leghorn WE strain |