| RRC ID |
84476
|
| Author |
Li XH, Duan JL, Ma JY, Liu XY, Sun XD, Wang Y, Tan MM, Yuan XZ.
|
| Title |
Probing the Surface Layer Modulation on Archaeal Mechanics and Adhesion at the Single-Cell Level.
|
| Journal |
Anal Chem
|
| Abstract |
Addressing the challenge of understanding how cellular interfaces dictate the mechanical resilience and adhesion of archaeal cells, this study demonstrates the role of the surface layer (S-layer) in methanogenic archaea. Using a combination of atomic force microscopy and single-cell force spectroscopy, we quantified the impact of S-layer disruption on cell morphology, mechanical properties, and adhesion capabilities. We demonstrate that the S-layer is crucial for maintaining cell morphology, where its removal induces significant cellular enlargement and deformation. Mechanical stability of the cell surface is substantially compromised upon S-layer disruption, as evidenced by decreased Young's modulus values. Adhesion experiments revealed that the S-layer primarily facilitates hydrophobic interactions, which are significantly reduced after its removal, affecting both cell-cell and cell-bubble interactions. Our findings illuminate the S-layer's fundamental role in methanogen architecture and provide a chemical understanding of archaeal cell surfaces, with implications for enhancing methane production in biotechnological applications.
|
| Volume |
96(22)
|
| Pages |
8981-8989
|
| Published |
2024-6-4
|
| DOI |
10.1021/acs.analchem.4c00038
|
| PMID |
38758609
|
| MeSH |
Archaea / chemistry
Archaea / metabolism
Cell Adhesion
Hydrophobic and Hydrophilic Interactions
Microscopy, Atomic Force*
Single-Cell Analysis*
Surface Properties*
|
| Resource |
| General Microbes |
JCM12185 |
