| RRC ID |
84494
|
| Author |
Zhang Y, Chen Y, Penttinen P, Wang X, Quan Y, Wen L, Yang M, Zhang X, Chen Q, Zhang L, Zhang J, Zhang X, Xu K.
|
| Title |
Ciceribacter sichuanensis sp. nov., a plant growth promoting rhizobacterium isolated from root nodules of soybean in Sichuan, China.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
The fast-growing rhizobia-like strains S101T and S153, isolated from root nodules of soybean (Glycine max) in Sichuan, People's Republic of China, underwent characterization using a polyphasic taxonomy approach. The strains exhibited growth at 20-40 °C (optimum, 28 °C), pH 4.0-10.0 (optimum, pH 7.0) and up to 2.0% (w/v) NaCl (optimum, 0.01%) on Yeast Mannitol Agar plates. The 16S rRNA gene of strain S101T showed 98.4% sequence similarity to the closest type strain, Ciceribacter daejeonense L61T. Major cellular fatty acids in strain S101T included summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c. The predominant quinone was ubiquinone-10. The polar lipids of strain S101T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethyl ethanolamine, phosphatidyl ethanolamine, amino phospholipid, unidentified phosphoglycolipid and unidentified amino-containing lipids. The DNA G + C contents of S101T and S153 were 61.1 and 61.3 mol%, respectively. Digital DNA-DNA hybridization relatedness and average nucleotide identity values between S101T and C. daejeonense L61T were 46.2% and 91.4-92.2%, respectively. In addition, strain S101T promoted the growth of soybean and carried nitrogen fixation genes in its genome, hinting at potential applications in sustainable agriculture. We propose that strains S101T and S153 represent a novel species, named Ciceribacter sichuanensis sp. nov., with strain S101T as the type strain (= CGMCC 1.61309 T = JCM 35649 T).
|
| Volume |
117(1)
|
| Pages |
46
|
| Published |
2024-3-1
|
| DOI |
10.1007/s10482-024-01941-5
|
| PII |
10.1007/s10482-024-01941-5
|
| PMID |
38427093
|
| MeSH |
Bacterial Typing Techniques
China
DNA, Bacterial / genetics
Ethanolamines
Fatty Acids / chemistry
Glycine max*
Humans
Phospholipids* / chemistry
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM35649 |
