| RRC ID |
84502
|
| 著者 |
Xie CJ, Yao L, Tang R, Han S, Yang S, Alwathnani H, Rensing C, Liu GH, Zhou SG.
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| タイトル |
Azotosporobacter soli gen. nov., sp. nov., a novel nitrogen-fixing bacterium isolated from paddy soil.
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| ジャーナル |
Antonie Van Leeuwenhoek
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| Abstract |
A nitrogen-fixing strain designated SG130T was isolated from paddy soil in Fujian Province, China. Strain SG130T was Gram-staining-negative, rod-shaped, and strictly anaerobic. Strain SG130T showed the highest 16S rRNA gene sequence similarities with the type strains Dendrosporobacter quercicolus DSM 1736T (91.7%), Anaeroarcus burkinensis DSM 6283T (91.0%) and Anaerospora hongkongensis HKU 15T (90.9%). Furthermore, the phylogenetic and phylogenomic analysis also suggested strain SG130T clustered with members of the family Sporomusaceae and was distinguished from other genera within this family. Growth of strain SG130T was observed at 25-45 °C (optimum 30 °C), pH 6.0-9.5 (optimum 7.0) and 0-1% (w/v) NaCl (optimum 0.1%). The quinones were Q-8 and Q-9. The polar lipids were phosphatidylserine (PS), phosphatidylethanolamine (PE), glycolipid (GL), phospholipid (PL) and an unidentified lipid (UL). The major fatty acids (> 10%) were iso-C13:0 3OH (26.6%), iso-C17:1 (15.6%) and iso-C15:1 F (11.4%). The genomic DNA G + C content was 50.7%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG130T and the most closely related type strain D. quercicolus DSM 1736T (ANI 68.0% and dDDH 20.3%) were both below the cut-off level for species delineation. The average amino acid identity (AAI) between strain SG130T and the most closely related type strain D. quercicolus DSM 1736T was 63.2%, which was below the cut-off value for bacterial genus delineation (65%). Strain SG130T possessed core genes (nifHDK) involved in nitrogen fixation, and nitrogenase activity (106.38 μmol C2H4 g-1 protein h-1) was examined using the acetylene reduction assay. Based on the above results, strain SG130T is confirmed to represent a novel genus of the family Sporomusaceae, for which the name Azotosporobacter soli gen. nov., sp. nov. is proposed. The type strain is SG130T (= GDMCC 1.3312T = JCM 35641T).
|
| 巻・号 |
117(1)
|
| ページ |
79
|
| 公開日 |
2024-5-17
|
| DOI |
10.1007/s10482-024-01978-6
|
| PII |
10.1007/s10482-024-01978-6
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| PMID |
38755437
|
| MeSH |
Bacterial Typing Techniques
Base Composition*
China
DNA, Bacterial* / genetics
Fatty Acids / analysis
Fatty Acids / metabolism
Nitrogen Fixation
Nitrogen-Fixing Bacteria / classification
Nitrogen-Fixing Bacteria / genetics
Nitrogen-Fixing Bacteria / isolation & purification
Nitrogen-Fixing Bacteria / metabolism
Phospholipids / analysis
Phylogeny*
RNA, Ribosomal, 16S* / genetics
Sequence Analysis, DNA
Soil Microbiology*
|
| リソース情報 |
| 一般微生物 |
JCM35641 |
