| RRC ID |
84508
|
| 著者 |
Hashimoto A, Shibata S, Hirooka Y, Ohkuma M.
|
| タイトル |
Phylogenetic and morphological re-evaluation of Camptophora.
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| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
The genetic variety and habitats of Camptophora species, generally known as black yeast, have not been clarified. In this study, we re-evaluated Camptophora based on morphological observations and phylogenetic analyses. Because prior investigations on Camptophora only included a few strains/specimens, 24 Camptophora-related strains were newly obtained from 13 leaf samples of various plant species to redefine the genetic and species concepts of Camptophora. Their molecular phylogenetic relationships were examined using small subunit nuclear ribosomal DNA (nSSU, 18S rDNA), the internal transcribed spacer (ITS) rDNA operon, the large subunit nuclear ribosomal DNA (LSU, 28S rDNA), β-tubulin, the second largest subunit of RNA polymerase II (rpb2), and mitochondrial small subunit DNA (mtSSU). Single- and multi-locus analyses using nSSU-ITS-LSU-rpb2-mtSSU revealed a robust phylogenetic relationship among Camptophora species within Chaetothyriaceae. Camptophora species could be distinguished from other chaetothyriaceous genera by their snake-shaped conidia with microcyclic conidiation and loosely interwoven mycelial masses. Based on the results of phylogenetic analyses, two undescribed lineages were recognized, and Ca. schimae was excluded from the genus. ITS sequence comparison with environmental DNA sequences revealed that the distribution of the genus is restricted to the Asia-Pacific region. Camptophora has been isolated or detected from abrupt sources, and this was attributed to its microcycle. The mechanisms driving genetic diversity within species are discussed with respect to their phyllosphere habitats.
|
| 巻・号 |
117(1)
|
| ページ |
109
|
| 公開日 |
2024-7-31
|
| DOI |
10.1007/s10482-024-01990-w
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| PII |
10.1007/s10482-024-01990-w
|
| PMID |
39083124
|
| MeSH |
Ascomycota / classification
Ascomycota / genetics
DNA, Fungal* / genetics
DNA, Ribosomal / genetics
DNA, Ribosomal Spacer / genetics
Phylogeny*
Plant Leaves / microbiology
RNA Polymerase II / genetics
Sequence Analysis, DNA
Spores, Fungal / classification
Spores, Fungal / cytology
Spores, Fungal / genetics
Tubulin / genetics
|
| リソース情報 |
| 一般微生物 |
JCM39274
JCM39276
JCM39278
JCM39275
JCM39279
JCM39277 |
