| RRC ID |
84532
|
| Author |
Narihara S, Chida S, Matsunaga N, Akimoto R, Akimoto M, Hagio A, Mori T, Nittami T, Sato M, Mun S, Kang H, Back JH, Takeda M.
|
| Title |
Taxonomic characterization of Sphaerotilus microaerophilus sp. nov., a sheath-forming microaerophilic bacterium of activated sludge origin.
|
| Journal |
Arch Microbiol
|
| Abstract |
A microaerophilic Gram-stain-negative bacilliform bacterial strain, FB-5 T, was isolated from activated sludge in Yokohama, Japan, that exhibited filamentous growth and formed a microtube (sheath). Cells were motile using a single polar flagellum. The optimum growth temperature and pH were 30 °C and 7.5, respectively. Strain FB-5 T was catalase-negative. Peptides and amino acids were utilized as energy and carbon sources. Sugars and organic acids were not utilized. Vitamin B12 enhanced the growth of strain FB-5 T. Sulfur-dependent lithotrophic growth was possible. Major respiratory quinone was UQ-8. Major fatty acids were C16:1ω7 and C16:0. The genomic DNA G + C content was 69.16%. Phylogenetic analysis of the 16S rRNA gene suggested that strain FB-5 T belongs to the genus Sphaerotilus. The close relatives were S. natans subsup. sulfidivorans and S. natans subsup. natans with 98.0% and 97.8% similarity based on the 16S rRNA gene analysis, respectively. The genome size (6.06 Mbp) was larger than that (4.39-5.07 Mbp) of the Sphaerotilus strains. The AAI values against the related strains ranged from 71.0 to 72.5%. The range of ANI values was 81.7 - 82.5%. In addition to these distinguishable features of the genome, the core genome and dDDH analyses suggested that this strain is a novel member of the genus Sphaerotilus. Based on its physiological properties and genomic features, strain FB-5 T is considered as a novel species of the genus Sphaerotilus, for which the name S. microaerophilus sp. nov. is proposed. The type strain is FB-5 T (= JCM 35424 T = KACC 23146 T).
|
| Volume |
206(6)
|
| Pages |
252
|
| Published |
2024-5-10
|
| DOI |
10.1007/s00203-024-03991-9
|
| PII |
10.1007/s00203-024-03991-9
|
| PMID |
38727820
|
| PMC |
PMC11087309
|
| MeSH |
Bacterial Typing Techniques
Base Composition*
DNA, Bacterial* / genetics
Fatty Acids* / analysis
Genome, Bacterial
Japan
Phylogeny*
RNA, Ribosomal, 16S* / genetics
Sequence Analysis, DNA
Sewage* / microbiology
|
| Resource |
| General Microbes |
JCM35424
JCM20382 |
