論文 - 詳細
| RRC ID | 84606 |
|---|---|
| 著者 | Khusnutdinova AN, Tran H, Devlekar S, Distaso MA, Kublanov IV, Skarina T, Stogios P, Savchenko A, Ferrer M, Golyshina OV, Yakunin AF, Golyshin PN. |
| タイトル | Moderately thermostable GH1 β-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5. |
| ジャーナル | FEMS Microbiol Ecol |
| Abstract |
Family GH1 glycosyl hydrolases are ubiquitous in prokaryotes and eukaryotes and are utilized in numerous industrial applications, including bioconversion of lignocelluloses. In this study, hyperacidophilic archaeon Cuniculiplasma divulgatum (S5T=JCM 30642T) was explored as a source of novel carbohydrate-active enzymes. The genome of C. divulgatum encodes three GH1 enzyme candidates, from which CIB12 and CIB13 were heterologously expressed and characterized. Phylogenetic analysis of CIB12 and CIB13 clustered them with β-glucosidases from genuinely thermophilic archaea including Thermoplasma acidophilum, Picrophilus torridus, Sulfolobus solfataricus, Pyrococcus furiosus, and Thermococcus kodakarensis. Purified enzymes showed maximal activities at pH 4.5-6.0 (CIB12) and 4.5-5.5 (CIB13) with optimal temperatures at 50°C, suggesting a high-temperature origin of Cuniculiplasma spp. ancestors. Crystal structures of both enzymes revealed a classical (α/β)8 TIM-barrel fold with the active site located inside the barrel close to the C-termini of β-strands including the catalytic residues Glu204 and Glu388 (CIB12), and Glu204 and Glu385 (CIB13). Both enzymes preferred cellobiose over lactose as substrates and were classified as cellobiohydrolases. Cellobiose addition increased the biomass yield of Cuniculiplasma cultures growing on peptides by 50%, suggesting that the cellobiohydrolases expand the carbon substrate range and hence environmental fitness of Cuniculiplasma. |
| 巻・号 | 100(9) |
| 公開日 | 2024-8-13 |
| DOI | 10.1093/femsec/fiae114 |
| PII | 7731458 |
| PMID | 39127612 |
| PMC | PMC11376072 |
| MeSH | Archaeal Proteins / chemistry Archaeal Proteins / genetics Archaeal Proteins / metabolism Enzyme Stability Hydrogen-Ion Concentration Phylogeny* Substrate Specificity Temperature beta-Glucosidase / genetics beta-Glucosidase / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM30642 |