RRC ID 84681
著者 Bai L, Paek J, Shin Y, Kim H, Kim SH, Shin JH, Kook JK, Chang YH.
タイトル Aerococcus kribbianus sp. nov., a facultatively anaerobic bacterium isolated from pig faeces.
ジャーナル Int J Syst Evol Microbiol
Abstract Two cocci-shaped, facultatively anaerobic, Gram-positive bacteria isolated from the faeces of a pig were designated as strains YH-aer221T and YH-aer222. Analysis of the 16S rRNA gene sequences revealed that the isolates were most closely related to Aerococcus suis JCM 18035T with 96.6 % similarity. The multi-locus sequence tree revealed that the isolates formed a sub-cluster adjacent to A. suis JCM 18035T. The average nucleotide identity values for the isolates and their most closely related strains were 71.8 and 71.7 %, respectively; and the digital DNA-DNA hybridization values for the isolates and their most closely related strains were 25.6 and 25.5 %, respectively. The main fatty acids were C18 : 1ω9c, C16 : 0 and C18 : 0. The cell wall contained the meso-diaminopimelic acid-based peptidoglycan. The two isolates shared the same metabolic pathways. Isolates YH-aer221T and YH-aer222 harboured the same CRISPR array with 33 and 46 spacers, respectively. Single-genome vs. metagenome analysis showed that the genomes of the isolates were not found in the available metagenome database. Given their chemotaxonomic, phenotypic and phylogenetic properties, YH-aer221T (= KCTC 25571T=JCM 35699T) and YH-aer222 (=KCTC 25573=JCM 35700) represent a novel taxon. The name Aerococcus kribbianus sp. nov. is proposed.
巻・号 74(2)
公開日 2024-2-1
DOI 10.1099/ijsem.0.006284
PMID 38415779
MeSH Aerococcus* Anaerobiosis Animals Bacteria, Anaerobic Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Feces Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Swine
リソース情報
一般微生物 JCM35699 JCM35700 JCM18035 JCM20461