Reference - Detail
| RRC ID | 84774 |
|---|---|
| Author | Xu Y, Zhao X, Jin J, Zhang R, Zhou C, Wang Z, Yao S, Wang X, Xiang W, Song J. |
| Title | Amycolatopsis melonis sp. nov., a novel protease-producing and cellulose-degrading actinobacterium isolated from soil. |
| Journal | Int J Syst Evol Microbiol |
| Abstract |
A novel protease-producing and cellulose-degrading actinobacterium, designated strain NEAU-NG30T, was isolated from a melon rhizosphere soil sample collected in Harbin, Heilongjiang Province, China, and established its status using a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-NG30T was closely related to Amycolatopsis bullii DSM 45802T (98.7%) and Amycolatopsis vancoresmycina DSM 44592T (98.3%). The phospholipid profile contained diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylinositol. The diagnostic sugars in cell hydrolysates were determined to be galactose and arabinose. Cell walls contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C15:0 and iso-C16:0. Meanwhile, genome analysis of sequences revealed a genome size of 9 338 250 bp and a DNA G+C content of 71.6%. In addition, the average nucleotide identity values and the level of digital DNA-DNA hybridization between strain NEAU-NG30T and its reference strains fall below the thresholds typically used for delineating prokaryote species. According to phenotypic, chemotaxonomic and genotypic studies, it is indicated that strain NEAU-NG30T is considered to be a novel species of the genus Amycolatopsis, for which the name Amycolatopsis melonis sp. nov. is proposed, with NEAU-NG30T (=MCCC 1K08677T=JCM 35654T) as the type strain. |
| Volume | 74(10) |
| Published | 2024-10-1 |
| DOI | 10.1099/ijsem.0.006559 |
| PMID | 39471071 |
| MeSH | Amycolatopsis* Bacterial Typing Techniques* Base Composition* Cellulose* / metabolism China Cucurbitaceae / microbiology DNA, Bacterial* / genetics Diaminopimelic Acid Fatty Acids* Nucleic Acid Hybridization* Peptide Hydrolases / genetics Peptide Hydrolases / metabolism Phospholipids* Phylogeny* RNA, Ribosomal, 16S* / genetics Rhizosphere* Sequence Analysis, DNA* Soil Microbiology* Vitamin K 2* / analogs & derivatives |
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| The most frequently cited source | Blogs |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM35654 |