| RRC ID |
84774
|
| 著者 |
Xu Y, Zhao X, Jin J, Zhang R, Zhou C, Wang Z, Yao S, Wang X, Xiang W, Song J.
|
| タイトル |
Amycolatopsis melonis sp. nov., a novel protease-producing and cellulose-degrading actinobacterium isolated from soil.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel protease-producing and cellulose-degrading actinobacterium, designated strain NEAU-NG30T, was isolated from a melon rhizosphere soil sample collected in Harbin, Heilongjiang Province, China, and established its status using a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-NG30T was closely related to Amycolatopsis bullii DSM 45802T (98.7%) and Amycolatopsis vancoresmycina DSM 44592T (98.3%). The phospholipid profile contained diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylinositol. The diagnostic sugars in cell hydrolysates were determined to be galactose and arabinose. Cell walls contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C15:0 and iso-C16:0. Meanwhile, genome analysis of sequences revealed a genome size of 9 338 250 bp and a DNA G+C content of 71.6%. In addition, the average nucleotide identity values and the level of digital DNA-DNA hybridization between strain NEAU-NG30T and its reference strains fall below the thresholds typically used for delineating prokaryote species. According to phenotypic, chemotaxonomic and genotypic studies, it is indicated that strain NEAU-NG30T is considered to be a novel species of the genus Amycolatopsis, for which the name Amycolatopsis melonis sp. nov. is proposed, with NEAU-NG30T (=MCCC 1K08677T=JCM 35654T) as the type strain.
|
| 巻・号 |
74(10)
|
| 公開日 |
2024-10-1
|
| DOI |
10.1099/ijsem.0.006559
|
| PMID |
39471071
|
| MeSH |
Amycolatopsis*
Bacterial Typing Techniques*
Base Composition*
Cellulose* / metabolism
China
Cucurbitaceae / microbiology
DNA, Bacterial* / genetics
Diaminopimelic Acid
Fatty Acids*
Nucleic Acid Hybridization*
Peptide Hydrolases / genetics
Peptide Hydrolases / metabolism
Phospholipids*
Phylogeny*
RNA, Ribosomal, 16S* / genetics
Rhizosphere*
Sequence Analysis, DNA*
Soil Microbiology*
Vitamin K 2* / analogs & derivatives
|
| リソース情報 |
| 一般微生物 |
JCM35654 |
