| RRC ID |
84831
|
| 著者 |
Kozawa T, Aoyagi H.
|
| タイトル |
Novel method for screening probiotic candidates tolerant to human gastrointestinal stress.
|
| ジャーナル |
J Microbiol Methods
|
| Abstract |
Tolerance to human gastrointestinal stressors is crucial for probiotics to exhibit their health benefits; however, there is no standardised method for screening their stress tolerance. In this study, we proposed a novel method for screening probiotic candidates tolerant to human gastrointestinal stress-gastrointestinal tolerance assay and culture (GTA-C) method-using black polyethylene terephthalate (PET) non-woven fabric as a scaffold to modify the specialized cellulose film (SCF) method. The modified SCF method showed excellent pH-based diffusion of medium components, had minimal effect on the growth of Escherichia coli K12, and improved the visibility of the colonies. Analysis of kimchi samples cultured using the SCF and modified SCF methods revealed that the modified method diversified the cultured bacteria. GTA in a simulated human fasting state using the modified SCF method showed that acid stress significantly affected the growth of four bacteria used as probiotics and that tolerance to acid stress may be species-dependent. Screening of probiotics in kimchi samples resulted in the identification of lactic acid bacteria tolerant to human gastrointestinal stress during fasting. Our results indicate that the modified SCF method (GTA-C method) is useful for screening probiotics resistant to the gastrointestinal environment during fasting.
|
| 巻・号 |
222
|
| ページ |
106945
|
| 公開日 |
2024-7-1
|
| DOI |
10.1016/j.mimet.2024.106945
|
| PII |
S0167-7012(24)00057-5
|
| PMID |
38729266
|
| MeSH |
Cellulose
Escherichia coli K12 / drug effects
Escherichia coli K12 / growth & development
Fasting
Fermented Foods / microbiology
Gastrointestinal Tract* / microbiology
Humans
Hydrogen-Ion Concentration
Probiotics*
Stress, Physiological*
|
| リソース情報 |
| 一般微生物 |
JCM1130
JCM1028
JCM1002
JCM1112
JCM6123 |
