Reference - Detail
| RRC ID | 84839 |
|---|---|
| Author | Mada T, Umeda A, Kodama A, Takamatsu D. |
| Title | The first isolation of Clostridium massiliodielmoense from a dead beef cow in Japan and pitfalls to be aware of in identifying the Clostridium species. |
| Journal | J Vet Med Sci |
| Abstract |
Clostridium sp. was detected in the organs of a cow with black watery diarrhea in Japan. Results identifying this species were inconsistent; Clostridium novyi type A infection was suggested by PCR assay targeting Clostridium fliC region (fliC-multiplex PCR), while 16S rRNA gene sequencing identified the isolated bacteria as Clostridium massiliodielmoense. Sequencing of fliC-multiplex PCR products from the isolates revealed the presence of fliC region in C. massiliodielmoense, which had 92.7% nucleotide similarity to that of C. novyi type A JCM 1406T, leading to the false positive detection of C. novyi by the PCR. This is the first C. massiliodielmoense isolation from clinical specimens, suggesting the need for further research on its pathogenicity and improvement in fliC-multiplex PCR. |
| Volume | 86(9) |
| Pages | 925-929 |
| Published | 2024-9-1 |
| DOI | 10.1292/jvms.24-0086 |
| PMID | 39010150 |
| PMC | PMC11422693 |
| MeSH | Animals Cattle Cattle Diseases* / diagnosis Cattle Diseases* / microbiology Clostridium* / genetics Clostridium* / isolation & purification Clostridium Infections* / diagnosis Clostridium Infections* / microbiology Clostridium Infections* / veterinary Diarrhea / microbiology Diarrhea / veterinary Japan Multiplex Polymerase Chain Reaction / veterinary RNA, Ribosomal, 16S* / genetics |
| Resource | |
| General Microbes | JCM1406 JCM1402 |