| RRC ID |
84901
|
| Author |
Nakai R, Kusada H, Sassa F, Makino A, Morigasaki S, Hayashi H, Takaya N, Tamaki H.
|
| Title |
Roseiterribacter gracilis gen. nov., sp. nov., a novel filterable alphaproteobacterium isolated from soil using a gel-filled microwell array device.
|
| Journal |
PLoS One
|
| Abstract |
Our previous studies indicate the abundant and diverse presence of yet-to-be-cultured microorganisms in the micropore-filtered fractions of various environmental samples. Here, we isolated a novel bacterium (designated as strain TMPK1T) from a 0.45-μm-filtered soil suspension by using a gel-filled microwell array device comprising 900 microwells and characterized its phylogenetic and physiological features. This strain showed low 16S rRNA gene sequence identities (<91%) and low average nucleotide identity values (<70%) to the closest validly described species, and belonged to a novel-family-level lineage within the order Rhodospirillales of Alphaproteobacteria. Strain TMPK1T exhibited small cell sizes (0.08-0.23 μm3) and had a high cyclopropane fatty acid content (>13%), and these characteristics were differentiated from other Rhodospirillales bacteria. A comprehensive habitability search using amplicon datasets suggested that TMPK1T and its close relatives are mainly distributed in soil and plant-associated environments. Based on these results, we propose that strain TMPK1T represents a novel genus and species named Roseiterribacter gracilis gen. nov., sp. nov. (JCM 34627T = KCTC 82790T). We also propose Roseiterribacteraceae fam. nov. to accommodate the genus Roseiterribacter.
|
| Volume |
19(6)
|
| Pages |
e0304366
|
| Published |
2024-1-1
|
| DOI |
10.1371/journal.pone.0304366
|
| PII |
PONE-D-23-36051
|
| PMID |
38857291
|
| PMC |
PMC11164329
|
| MeSH |
Alphaproteobacteria / genetics
Alphaproteobacteria / isolation & purification
DNA, Bacterial / genetics
Fatty Acids / analysis
Phylogeny*
RNA, Ribosomal, 16S* / genetics
Soil Microbiology*
|
| Resource |
| General Microbes |
JCM34627 |
