論文 - 詳細
| RRC ID | 84901 |
|---|---|
| 著者 | Nakai R, Kusada H, Sassa F, Makino A, Morigasaki S, Hayashi H, Takaya N, Tamaki H. |
| タイトル | Roseiterribacter gracilis gen. nov., sp. nov., a novel filterable alphaproteobacterium isolated from soil using a gel-filled microwell array device. |
| ジャーナル | PLoS One |
| Abstract |
Our previous studies indicate the abundant and diverse presence of yet-to-be-cultured microorganisms in the micropore-filtered fractions of various environmental samples. Here, we isolated a novel bacterium (designated as strain TMPK1T) from a 0.45-μm-filtered soil suspension by using a gel-filled microwell array device comprising 900 microwells and characterized its phylogenetic and physiological features. This strain showed low 16S rRNA gene sequence identities (<91%) and low average nucleotide identity values (<70%) to the closest validly described species, and belonged to a novel-family-level lineage within the order Rhodospirillales of Alphaproteobacteria. Strain TMPK1T exhibited small cell sizes (0.08-0.23 μm3) and had a high cyclopropane fatty acid content (>13%), and these characteristics were differentiated from other Rhodospirillales bacteria. A comprehensive habitability search using amplicon datasets suggested that TMPK1T and its close relatives are mainly distributed in soil and plant-associated environments. Based on these results, we propose that strain TMPK1T represents a novel genus and species named Roseiterribacter gracilis gen. nov., sp. nov. (JCM 34627T = KCTC 82790T). We also propose Roseiterribacteraceae fam. nov. to accommodate the genus Roseiterribacter. |
| 巻・号 | 19(6) |
| ページ | e0304366 |
| 公開日 | 2024-1-1 |
| DOI | 10.1371/journal.pone.0304366 |
| PII | PONE-D-23-36051 |
| PMID | 38857291 |
| PMC | PMC11164329 |
| MeSH | Alphaproteobacteria / genetics Alphaproteobacteria / isolation & purification DNA, Bacterial / genetics Fatty Acids / analysis Phylogeny* RNA, Ribosomal, 16S* / genetics Soil Microbiology* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM34627 |