| RRC ID |
84904
|
| 著者 |
Ma JY, Yan Z, Sun XD, Jiang YQ, Duan JL, Feng LJ, Zhu FP, Liu XY, Xia PF, Yuan XZ.
|
| タイトル |
A hybrid photocatalytic system enables direct glucose utilization for methanogenesis.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
Integration of methanogenic archaea with photocatalysts presents a sustainable solution for solar-driven methanogenesis. However, maximizing CH4 conversion efficiency remains challenging due to the intrinsic energy conservation and strictly restricted substrates of methanogenic archaea. Here, we report a solar-driven biotic-abiotic hybrid (biohybrid) system by incorporating cadmium sulfide (CdS) nanoparticles with a rationally designed methanogenic archaeon Methanosarcina acetivorans C2A, in which the glucose synergist protein and glucose kinase, an energy-efficient route for glucose transport and phosphorylation from Zymomonas mobilis, were implemented to facilitate nonnative substrate glucose for methanogenesis. We demonstrate that the photo-excited electrons facilitate membrane-bound electron transport chain, thereby augmenting the Na+ and H+ ion gradients across membrane to enhance adenosine triphosphate (ATP) synthesis. Additionally, this biohybrid system promotes the metabolism of pyruvate to acetyl coenzyme A (AcCoA) and inhibits the flow of AcCoA to the tricarboxylic acid (TCA) cycle, resulting in a 1.26-fold augmentation in CH4 production from glucose-derived carbon. Our results provide a unique strategy for enhancing methanogenesis through rational biohybrid design and reprogramming, which gives a promising avenue for sustainably manufacturing value-added chemicals.
|
| 巻・号 |
121(4)
|
| ページ |
e2317058121
|
| 公開日 |
2024-1-23
|
| DOI |
10.1073/pnas.2317058121
|
| PMID |
38232281
|
| PMC |
PMC10823229
|
| MeSH |
Adenosine Triphosphate* / metabolism
Biological Transport
Electron Transport
Energy Metabolism
Methane* / metabolism
Methanosarcina / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM12185 |
