Reference - Detail
| RRC ID | 84932 |
|---|---|
| Author | Podosokorskaya OA, Elcheninov AG, Klyukina AA, Merkel AY. |
| Title | Ignisphaera cupida sp. nov., a hyperthermophilic hydrolytic archaeon from a hot spring of Uzon (Kamchatka), and emended description of the genus Ignisphaera. |
| Journal | Syst Appl Microbiol |
| Abstract |
A novel strictly anaerobic hyperthermophilic archaeon, strain 4213-coT, was isolated from a terrestrial hot spring in the Uzon Caldera, Kamchatka (Russian Federation). Coccoid cells were present singly, in pairs, or aggregates, and occasionally were motile. The strain grew at 75-100 °C and within a pH range of 5.4-8.2 with the optimum at 92 °C and pH 6.4-6.7. Strain 4213-coT was a chemoorganoheterotroph, growing on proteinaceous substrates and mono-, di- and polysaccharides (starch, guar gum, xanthan gum). It did not require sodium chloride for growth. The complete genome of strain 4213-coT was 1.74 Mbp in size; its G+C content was 36.18 %. Genome analysis allowed to identify 25 genes encoding glycosidases involved in polysaccharide hydrolysis as well as genes of ADP-forming acetate-CoA ligase, lactate dehydrogenase and two [NiFe] hydrogenases responsible for acetate, lactate and hydrogen formation during fermentation. Moreover gene cluster encoding archaellum subunits was found. According to the phylogenomic analysis strain 4213-coT formed a species-level phylogenetic lineage within Ignisphaera genus. Our phylogenomic analysis also supports the delineation of the Ignisphaera genus into a separate family Ignisphaeraceae, as recently published. Here we propose a novel species Ignisphaera cupida, sp. nov. with type strain 4213-coT (=JCM 39446T=VKM B-3715T=UQM 41593T). Ecogenomic analysis showed that representatives of the Ignisphaera are thermophilic archaea, the majority of them were found in terrestrial hot springs and deep-sea hydrothermal vents. This study allowed a better understanding of physiology and ecology of Ignisphaeraceae - a rather understudied archaeal group. |
| Volume | 47(5) |
| Pages | 126541 |
| Published | 2024-9-1 |
| DOI | 10.1016/j.syapm.2024.126541 |
| PII | S0723-2020(24)00055-9 |
| PMID | 39084153 |
| MeSH | Archaea / classification Archaea / genetics Archaea / isolation & purification Base Composition* DNA, Archaeal / chemistry DNA, Archaeal / genetics Genome, Archaeal Hot Springs* / microbiology Hot Temperature Hydrogen-Ion Concentration Hydrolysis Phylogeny* RNA, Ribosomal, 16S / genetics Russia Sequence Analysis, DNA |
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM39446 |