論文 - 詳細
| RRC ID | 85071 |
|---|---|
| 著者 | Lee HE, Park SJ, Huh JW, Imai H, Kim HS. |
| タイトル | The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB. |
| ジャーナル | Sci Rep |
| Abstract |
Transposable elements (TEs) are DNA sequences that cut or introduced into the genome, and they represent a massive portion of the human genome. TEs generate a considerable number of microRNAs (miRNAs) are derived from TEs (MDTEs). Numerous miRNAs are related to cancer, and hsa-miRNA-625 is a well-known oncomiR derived from long interspersed nuclear elements (LINEs). The relative expression of hsa-miRNA-625-5p differs in humans, chimpanzees, crab-eating monkeys, and mice, and four primers were designed against the 3'UTR of GATAD2B to analyze the different quantities of canonical binding sites and the location of miRNA binding sites. Luciferase assay was performed to score for the interaction between hsa-miRNA-625 and the 3'UTR of GATAD2B, while blocking NF-κB. In summary, the different numbers of canonical binding sites and the locations of miRNA binding sites affect gene expression, and NF-κB induces the enhancer activity of hsa-miRNA-625-5p by sharing the binding sites. |
| 巻・号 | 11(1) |
| ページ | 3139 |
| 公開日 | 2021-2-4 |
| DOI | 10.1038/s41598-021-82735-x |
| PII | 10.1038/s41598-021-82735-x |
| PMID | 33542430 |
| PMC | PMC7862687 |
| MeSH | 3' Untranslated Regions A549 Cells Animals Base Pairing Computational Biology / methods DNA Primers / chemical synthesis DNA Primers / metabolism DNA Transposable Elements* Enhancer Elements, Genetic* Genes, Reporter Genome* Humans Long Interspersed Nucleotide Elements* Luciferases / genetics Luciferases / metabolism Macaca fascicularis Mice MicroRNAs / genetics* MicroRNAs / metabolism NF-kappa B / genetics* NF-kappa B / metabolism Pan troglodytes Repressor Proteins / genetics* Repressor Proteins / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| GAIN | human western chimpanzee crab-eating monkey |