論文 - 詳細
| RRC ID | 85216 |
|---|---|
| 著者 | Mi J, Liu KC, Andersson O. |
| タイトル | Decoding pancreatic endocrine cell differentiation and β cell regeneration in zebrafish. |
| ジャーナル | Sci Adv |
| Abstract |
In contrast to mice, zebrafish have an exceptional yet elusive ability to replenish lost β cells in adulthood. Understanding this framework would provide mechanistic insights for β cell regeneration, which may be extrapolated to humans. Here, we characterize a krt4-expressing ductal cell type, which is distinct from the putative Notch-responsive cells, showing neogenic competence and giving rise to the majority of endocrine cells during postembryonic development. Furthermore, we demonstrate a marked ductal remodeling process featuring a Notch-responsive to krt4+ luminal duct transformation during late development, indicating several origins of krt4+ ductal cells displaying similar transcriptional patterns. Single-cell transcriptomics upon a series of time points during β cell regeneration unveil a previously unrecognized dlb+ transitional endocrine precursor cell, distinct regulons, and a differentiation trajectory involving cellular shuffling through differentiation and dedifferentiation dynamics. These results establish a model of zebrafish pancreatic endocrinogenesis and highlight key values of zebrafish for translational studies of β cell regeneration. |
| 巻・号 | 9(33) |
| ページ | eadf5142 |
| 公開日 | 2023-8-18 |
| DOI | 10.1126/sciadv.adf5142 |
| PMID | 37595046 |
| PMC | PMC10438462 |
| MeSH | Animals Cell Differentiation Endocrine Cells* Humans Insulin-Secreting Cells* Mice Regeneration Zebrafish |
| IF | 13.117 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:GFP |