| RRC ID |
85231
|
| Author |
Matthes MS, Yun N, Luichtl M, Büschges U, Fiesselmann BS, Strickland B, Lehnardt MS, Schneitz K, Michel K, Torres Ruiz RA.
|
| Title |
Distinct domains of ENHANCER OF PINOID hold information for its polarization required for auxin-mediated cotyledon and flower development in Arabidopsis.
|
| Journal |
PLoS Genet
|
| Abstract |
The Arabidopsis ENHANCER OF PINOID (ENP) protein and the AGC-kinase PINOID (PID) synergistically impact on polarization and function of the auxin transporter PIN-FORMED1 (PIN1) required for plant leaf and flower organ development. ENP offers a PID-independent input for PIN-function since enp pid double mutants lead to cotyledon- and flower-less plants in contrast to pid single mutants, which develop cotyledons and abnormal albeit fertile flowers. This indicates that ENP, which depicts a similar polar localization as PIN1, is a potential interactor of PINs including PIN1. Here we show that the modular structure of ENP predicted by AlphaFold separates the capability for its own cellular polarization and its function linked to polar PIN1 activity. The part of ENP from aa1 to aa470 is subdivided into three structured domains. They are supportive and/or essential for cellular polarity. In contrast, the C-terminus, which is an intrinsically disordered region (IDR), is completely dispensable for polarity but essential for ENP-mediated PIN1-function. FLIM-FRET shows ENP to be closely associated with the plasma membrane and its IDR to significantly interact with PINs. Moreover, the modification status of two prominent phosphorylation sites in the IDR determines ENPs stability and its capability in supporting PIN1. Our results show ENP to be an element in the assumed PIN-multiprotein complex and explain its impact on PID-independent PIN1 activity.
|
| Volume |
21(6)
|
| Pages |
e1011217
|
| Published |
2025-6-1
|
| DOI |
10.1371/journal.pgen.1011217
|
| PII |
PGENETICS-D-24-00274
|
| PMID |
40549793
|
| PMC |
PMC12201645
|
| MeSH |
Arabidopsis* / genetics
Arabidopsis* / growth & development
Arabidopsis* / metabolism
Arabidopsis Proteins* / chemistry
Arabidopsis Proteins* / genetics
Arabidopsis Proteins* / metabolism
Cell Polarity / genetics
Cotyledon* / genetics
Cotyledon* / growth & development
Cotyledon* / metabolism
Flowers* / genetics
Flowers* / growth & development
Flowers* / metabolism
Gene Expression Regulation, Plant
Indoleacetic Acids* / metabolism
Membrane Transport Proteins* / genetics
Membrane Transport Proteins* / metabolism
Mutation
Protein Domains
Protein Serine-Threonine Kinases
|
| IF |
5.175
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda08292
pda10515 |
