論文 - 詳細
| RRC ID | 85483 |
|---|---|
| 著者 | Getz LJ, Robinson OS, Thomas NA. |
| タイトル | Functional genomics of chitin degradation by Vibrio parahaemolyticus reveals finely integrated metabolic contributions to support environmental fitness. |
| ジャーナル | PLoS Genet |
| Abstract |
Vibrio species are marine prokaryotes that inhabit diverse ecological niches, colonizing abiotic and biotic surfaces. These bacteria are vital players in the global carbon cycle, assimilating billions of tonnes of chitin for carbon (and nitrogen) metabolites. Many bacterial proteins involved in the process-including chitinases, sugar transporters, and modifying enzymes-have been well studied. However, the genetic functional interplay and key drivers of Vibrio competitive survival in the presence of chitin as the dominant carbon source is not understood. To address this question, we carried out transposon sequencing (Tn-seq) to determine the genetic fitness of Vibrio parahaemolyticus mutants grown on chitin as a sole carbon source. Along with validating known Vibrio genes associated with chitin metabolism, our data newly identified vital roles for an unclassified OprD-like import chitoporin and a HexR family transcriptional regulator. Furthermore, we functionally implicated HexR in regulating multiple physiological processes involved in V. parahaemolyticus environmental survival including carbon assimilation and cell growth, biofilm formation, and cell motility. Under nutrient limiting conditions, our data revealed a requirement for HexR in filamentous cell morphology, a critical trait for V. parahaemolyticus environmental fitness. Therefore, a vital import porin and genomic regulation mediated by HexR support multiple physiological processes for Vibrio chitinolytic growth and environmental fitness. |
| 巻・号 | 21(3) |
| ページ | e1011370 |
| 公開日 | 2025-3-1 |
| DOI | 10.1371/journal.pgen.1011370 |
| PII | PGENETICS-D-24-00797 |
| PMID | 40029889 |
| PMC | PMC11906056 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism Biofilms / growth & development Carbon / metabolism Chitin* / genetics Chitin* / metabolism Chitinases / genetics Chitinases / metabolism DNA Transposable Elements / genetics Gene Expression Regulation, Bacterial Genetic Fitness Genomics / methods Transcription Factors / genetics Transcription Factors / metabolism Vibrio parahaemolyticus* / genetics Vibrio parahaemolyticus* / metabolism |
| IF | 5.175 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原細菌 | JNBP_30766 |