| RRC ID |
85511
|
| 著者 |
Díaz Suárez L, Inagaki H, Hirata H, Akimoto Y, Sakakibayashi K, Seino H, Ito M, Cao L, Nakabayashi K, Kato K, Onishi K.
|
| タイトル |
Fine-tuning of heading time by earliness per se effect due to multi-allelic variants in VRN-B3 locus of hexaploid wheat.
|
| ジャーナル |
Planta
|
| Abstract |
Wheat VRN-B3 contains multi-allelic variants conferring earliness per se effects and can generate a new allele by recombining multiple alleles, highlighting its importance for the fine-tuning of heading time. Fine-tuning of heading time is required for breeding well-adapted varieties of wheat in regional environments. The VRN-B3 locus, which encodes the FT-B1 gene, is known as a vernalization gene. In this study, we analyzed two alleles in the VRN-B3 region: QHt-7B_Zen of a Japanese variety (Zenkouji-komugi) and QHt-7B_spelt of a spelt wheat strain (st. Rumania). Phenotypic evaluation of near-isogeneic lines (NILs) of 'Chinese Spring' (CS) under long-day (16 h) conditions showed that QHt-7B_Zen and QHt-7B_spelt conferred approximately 3.9 d earlier and 3.0 d later heading time compared with CS, respectively. Differences in heading times among NILs were also observed for fully vernalized plants under long-day conditions, indicating their earliness per se effect. Both QTLs behaved as single genes with incomplete dominant effects, and fine-mapping showed that FT-B1 was responsible for heading time. Droplet digital PCR analysis revealed that QHt-7B_Zen contained three copies of FT-B1, similar to the CS. QHt-7B_spelt had one FT-B1 copy with 14 substitutions, a 15 bp insertion in the 4.8 kb promoter region, and one amino acid substitution in the third exon, which could be designated as a novel allele, Vrn-B3f. Furthermore, a new allele with two FT-B1 copies conferring an intermediate heading time between the parents was created by the recombination of FT-B1 copies of CS and NIL for QHt-7B_spelt. Our findings indicate that fine-tuning heading time is possible through the versatility of the VRN-B3 locus, which can generate multi-allelic variants that have both vernalization and earliness per se effects in wheat.
|
| 巻・号 |
261(5)
|
| ページ |
97
|
| 公開日 |
2025-3-28
|
| DOI |
10.1007/s00425-025-04674-5
|
| PII |
10.1007/s00425-025-04674-5
|
| PMID |
40153070
|
| MeSH |
Alleles
Chromosome Mapping
Flowers / genetics
Flowers / growth & development
Flowers / physiology
Genes, Plant / genetics
Phenotype
Plant Proteins* / genetics
Plant Proteins* / metabolism
Polyploidy
Quantitative Trait Loci / genetics
Triticum* / genetics
Triticum* / growth & development
Triticum* / physiology
|
| IF |
3.39
|
| リソース情報 |
| コムギ |
KT020-113
KT019-002 |
