| RRC ID |
85519
|
| 著者 |
Bakosova A, Cipak L, Mayerova N, Krol K, Benko Z, Pitelova A, Kolesar P, Piatrova D, Smondrkova M, Maresova A, Molnarova L, Cipakova I, Altmannova V, Bellova J, Barath P, Prevorovsky M, Palecek J, Krejci L, Gregan J, Skoneczna A, Polakova SB.
|
| タイトル |
Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Helicases and endonucleases play crucial roles in genome maintenance by unwinding or cleaving various forms of DNA and RNA structures in order to facilitate essential biological processes, such as DNA replication and recombination. Here, we identified fission yeast Dbl2 as a potential interactor of several complexes that exhibit either helicase or endonuclease activity, namely Fml1-MHF, SCFFbh1, Rqh1-Top3-Rmi1, and Mus81-Eme1. In vitro, Dbl2 binds to DNA, with a preference for branched molecules, such as D-loops, mobile Holliday junctions, and fork structures, making it a good candidate to play a central role in modulating the activity of helicases and endonucleases during replication and recombination repair. Previously, we showed that Dbl2 recruits Fbh1 to the ongoing homologous recombination sites, affecting the Rad51-nucleofilament. In this study, we determined that deleting dbl2 in an fbh1Δ background did not increase sensitivity to DNA-damaging agents or the frequency of Tf2 ectopic recombination. Therefore, Dbl2 and Fbh1 might be involved in the same molecular pathway, maintaining genome integrity by hindering ectopic recombination at repetitive elements.
|
| 巻・号 |
15(1)
|
| ページ |
21895
|
| 公開日 |
2025-7-1
|
| DOI |
10.1038/s41598-025-08626-7
|
| PII |
10.1038/s41598-025-08626-7
|
| PMID |
40594858
|
| PMC |
PMC12217596
|
| MeSH |
DNA Damage
DNA Helicases* / genetics
DNA Helicases* / metabolism
DNA Replication
DNA, Fungal / genetics
DNA, Fungal / metabolism
DNA-Binding Proteins / metabolism
Endonucleases* / genetics
Endonucleases* / metabolism
Protein Binding
Repetitive Sequences, Nucleic Acid*
Schizosaccharomyces* / genetics
Schizosaccharomyces* / metabolism
Schizosaccharomyces pombe Proteins* / genetics
Schizosaccharomyces pombe Proteins* / metabolism
|
| IF |
3.998
|
| リソース情報 |
| 遺伝子材料 |
pDUAL-YFH1 (RDB06191)
SPYFH38F02 (SPW095322)
SpYFH35F02 (SPW094122) |
