| RRC ID |
85586
|
| 著者 |
Taoka H, Murakawa T, Kawaguchi K, Koizumi M, Kaminishi T, Sakamaki Y, Tanaka K, Harada A, Inoue K, Kanki T, Ohkawa Y, Fujita N.
|
| タイトル |
Transcriptional dynamics uncover the role of BNIP3 in mitophagy during muscle remodeling in Drosophila.
|
| ジャーナル |
Elife
|
| Abstract |
Differentiated muscle cells contain myofibrils and well-organized organelles, enabling powerful contractions. Muscle cell reorganization occurs in response to various physiological stimuli; however, the mechanisms behind this remodeling remain enigmatic due to the lack of a genetically trackable system. Previously, we reported that a subset of larval muscle cells is remodeled into adult abdominal muscle through an autophagy-dependent mechanism in Drosophila. To unveil the underlying mechanisms of this remodeling, we performed a comparative time-course RNA-seq analysis of isolated muscle cells with or without autophagy. It revealed both transcriptional dynamics independent of autophagy and highlighted the significance of BNIP3-mediated mitophagy in muscle remodeling. Mechanistically, we found that BNIP3 recruits autophagic machinery to mitochondria through its LC3-interacting motif and minimal essential region, which interact with Atg8a and Atg18a, respectively. Loss of BNIP3 leads to a substantial accumulation of larval mitochondria, ultimately impairing muscle remodeling. In summary, this study demonstrates that BNIP3-dependent mitophagy is critical for orchestrating the dynamic process of muscle remodeling.
|
| 巻・号 |
14
|
| 公開日 |
2025-8-13
|
| DOI |
10.7554/eLife.105834
|
| PII |
105834
|
| PMID |
40801807
|
| PMC |
PMC12349898
|
| MeSH |
Animals
Autophagy-Related Proteins / metabolism
Drosophila Proteins* / genetics
Drosophila Proteins* / metabolism
Drosophila melanogaster* / genetics
Larva / genetics
Membrane Proteins* / genetics
Membrane Proteins* / metabolism
Mitochondria / metabolism
Mitochondrial Proteins* / genetics
Mitochondrial Proteins* / metabolism
Mitophagy* / genetics
Transcription, Genetic*
|
| IF |
7.08
|
| リソース情報 |
| ショウジョウバエ |
HMS01349
HMJ21292
HMJ22436
DGRC#117016 |
