論文 - 詳細
| RRC ID | 85586 |
|---|---|
| 著者 | Taoka H, Murakawa T, Kawaguchi K, Koizumi M, Kaminishi T, Sakamaki Y, Tanaka K, Harada A, Inoue K, Kanki T, Ohkawa Y, Fujita N. |
| タイトル | Transcriptional dynamics uncover the role of BNIP3 in mitophagy during muscle remodeling in Drosophila. |
| ジャーナル | Elife |
| Abstract |
Differentiated muscle cells contain myofibrils and well-organized organelles, enabling powerful contractions. Muscle cell reorganization occurs in response to various physiological stimuli; however, the mechanisms behind this remodeling remain enigmatic due to the lack of a genetically trackable system. Previously, we reported that a subset of larval muscle cells is remodeled into adult abdominal muscle through an autophagy-dependent mechanism in Drosophila. To unveil the underlying mechanisms of this remodeling, we performed a comparative time-course RNA-seq analysis of isolated muscle cells with or without autophagy. It revealed both transcriptional dynamics independent of autophagy and highlighted the significance of BNIP3-mediated mitophagy in muscle remodeling. Mechanistically, we found that BNIP3 recruits autophagic machinery to mitochondria through its LC3-interacting motif and minimal essential region, which interact with Atg8a and Atg18a, respectively. Loss of BNIP3 leads to a substantial accumulation of larval mitochondria, ultimately impairing muscle remodeling. In summary, this study demonstrates that BNIP3-dependent mitophagy is critical for orchestrating the dynamic process of muscle remodeling. |
| 巻・号 | 14 |
| 公開日 | 2025-8-13 |
| DOI | 10.7554/eLife.105834 |
| PII | 105834 |
| PMID | 40801807 |
| PMC | PMC12349898 |
| MeSH | Animals Autophagy-Related Proteins / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Larva / genetics Membrane Proteins* / genetics Membrane Proteins* / metabolism Mitochondria / metabolism Mitochondrial Proteins* / genetics Mitochondrial Proteins* / metabolism Mitophagy* / genetics Transcription, Genetic* |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | HMS01349 HMJ21292 HMJ22436 DGRC#117016 |