RRC ID 856
Author Kaga N, Umitsuki G, Clark DP, Nagai K, Wachi M.
Title Extensive overproduction of the AdhE protein by rng mutations depends on mutations in the cra gene or in the Cra-box of the adhE promoter.
Journal Biochem. Biophys. Res. Commun.
Abstract Escherichia coli RNase G encoded by the rng gene is involved in degradation of adhE mRNA. Overproduction of the AdhE protein by rng mutants was found to depend on the genetic background of strains derived from DC272 (adhC81) or MC1061. We found that DC272 carried a point mutation in the Cra-binding site of the adhE promoter. The Cra protein encoded by the cra gene is known to act as a repressor of adhE. P1-phage-mediated transduction and lacZ fusion analysis with the mutant adhE promoter confirmed that this mutation is responsible for overproduction. On the other hand, Southern hybridization revealed that MC1061 had a 0.85-kb deletion of the cra gene. Overproduction of AdhE in the MC1061 background was reversed to the wild-type levels by introduction of a plasmid carrying the cra(+) gene. These results indicated that expression of the adhE gene was regulated transcriptionally by Cra and posttranscriptionally by RNase G.
Volume 295(1)
Pages 92-7
Published 2002-7-5
PII S0006-291X(02)00639-3
PMID 12083772
MeSH Alcohol Dehydrogenase / biosynthesis Alcohol Dehydrogenase / genetics* Aldehyde Oxidoreductases / biosynthesis Aldehyde Oxidoreductases / genetics* Bacterial Proteins / genetics* Bacterial Proteins / metabolism Base Sequence Binding Sites Endoribonucleases / genetics* Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins* Gene Deletion Gene Expression Regulation, Bacterial Molecular Sequence Data Multienzyme Complexes / biosynthesis Multienzyme Complexes / genetics* Mutation Promoter Regions, Genetic* Regulatory Sequences, Nucleic Acid Repressor Proteins / genetics* Repressor Proteins / metabolism Species Specificity
IF 2.559
Times Cited 11
Prokaryotes E. coli ?CV-48(MC1061) ME8481(BW7622)