論文 - 詳細
| RRC ID | 85683 |
|---|---|
| 著者 | Iwasaki T, Shimoda M, Kanayama H, Kawano T. |
| タイトル | Plasmodium falciparum histidine-rich protein 2 exhibits cell penetration and cytotoxicity with autophagy dysfunction. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Plasmodium falciparum is a major cause of severe malaria. This protozoan infects human red blood cells and secretes large quantities of histidine-rich protein 2 (PfHRP2) into the bloodstream, making it a well-known diagnostic marker. Here, however, we identified PfHRP2 as a pathogenic factor produced by P. falciparum. PfHRP2 showed cell penetration and cytotoxicity against various human cells. PfHRP2 also exhibited significant cytotoxicity at concentrations found in P. falciparum-infected patients' blood (90-100 n m). We also showed that PfHRP2 binds to Ca2+ ions, localizes to intracellular lysosomes, increases lysosomal Ca2+ levels, and inhibits the basal level of autophagy by preventing autolysosome formation. Furthermore, the Ca2+-dependent cytotoxicity of PfHRP2 was suppressed by the metal ion chelator ethylenediaminetetraacetic acid. In summary, our findings suggest PfHRP2 as a crucial pathogenic factor produced by P. falciparum and its mode of action. Overall, this study provides preliminary insights into P. falciparum malaria pathogenesis. |
| 巻・号 | 89(4) |
| ページ | 548-561 |
| 公開日 | 2025-3-24 |
| DOI | 10.1093/bbb/zbae209 |
| PII | 7945203 |
| PMID | 39777447 |
| MeSH | Antigens, Protozoan* / metabolism Antigens, Protozoan* / toxicity Autophagy* / drug effects Calcium / metabolism Edetic Acid / pharmacology Erythrocytes / parasitology Humans Lysosomes / drug effects Lysosomes / metabolism Malaria, Falciparum / parasitology Plasmodium falciparum* / metabolism Plasmodium falciparum* / pathogenicity Protozoan Proteins* / metabolism Protozoan Proteins* / toxicity |
| IF | 1.516 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HT1080(RCB1956) |