論文 - 詳細
| RRC ID | 86129 |
|---|---|
| 著者 | Horie M, Takumida H, Koba H, Ueda T, Tanaka H, Suzuki M, Ito Y, Ito A, Kondo M, Suzuki HI, Matsumoto I, Yano S, Saito A, Maeda D. |
| タイトル | Characteristic miRNA profiles represent clinicopathological diversity of small cell lung cancer. |
| ジャーナル | J Pathol |
| Abstract |
Small cell lung cancer (SCLC) is classified into distinct molecular subtypes based on the expression patterns of four transcription regulators: achaete-scute homolog 1 (ASCL1), neuronal differentiation 1 (NEUROD1), POU class 2 homeobox 3 (POU2F3), and yes-associated protein 1 (YAP1). MicroRNAs (miRNAs) play critical roles in cancer cellular processes but their subtype-specific implications in SCLC remain underexplored. Out of 46 surgically resected SCLC samples, miRNA visualization through in situ hybridization identified high expression of miR-375 in the ASCL1, NEUROD1, and ASCL1/NEUROD1 subtypes, and miR-9-5p in the POU2F3 subtype. Comprehensive enhancer profiling using SCLC cell lines indicated that miR-375 and miR-9-5p were regulated by super-enhancers in a subtype-specific manner. Multiplex immunohistochemistry by imaging mass cytometry found that the miR-9-5p-high SCLC was characterized by a higher stromal area ratio, increased numbers of CD8+ T cells and CD163- macrophages in the intra-tumoral area, and an increased number of plasma cells in the stromal area, as compared with the miR-9-5p-low SCLC. Finally, clinicopathological analysis revealed that the miR-375-high SCLC was associated with YAP1 downregulation, increased serum pro-gastrin-releasing peptide levels, and poor prognosis. These findings highlight the critical role of super-enhancer-related miRNAs in the diversity of SCLC, and underscore the potential for novel diagnostic and prognostic biomarkers based on these subtype-specific miRNAs. © 2025 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. |
| 巻・号 | 267(2) |
| ページ | 168-180 |
| 公開日 | 2025-10-1 |
| DOI | 10.1002/path.6458 |
| PMID | 40842416 |
| PMC | PMC12438024 |
| MeSH | Aged Basic Helix-Loop-Helix Transcription Factors Biomarkers, Tumor* / genetics Biomarkers, Tumor* / metabolism Cell Line, Tumor Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Lung Neoplasms* / genetics Lung Neoplasms* / pathology Male MicroRNAs* / genetics MicroRNAs* / metabolism Middle Aged Small Cell Lung Carcinoma* / genetics Small Cell Lung Carcinoma* / pathology YAP-Signaling Proteins |
| IF | 6.021 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Lu-134-A(RCB0466) |