論文 - 詳細
| RRC ID | 86177 |
|---|---|
| 著者 | Sakamoto K, Yamamoto Y, Inaba H, Matsuura K. |
| タイトル | Strategy toward In-Cell Self-Assembly of an Artificial Viral Capsid from a Fluorescent Protein-Modified β-Annulus Peptide. |
| ジャーナル | ACS Synth Biol |
| Abstract |
In-cell self-assembly of natural viral capsids is an event that can be visualized under transmission electron microscopy (TEM) observations. By mimicking the self-assembly of natural viral capsids, various artificial protein- and peptide-based nanocages were developed; however, few studies have reported the in-cell self-assembly of such nanocages. Our group developed a β-Annulus peptide that can form a nanocage called artificial viral capsid in vitro, but in-cell self-assembly of the capsid has not been achieved. Here, we designed an artificial viral capsid decorated with a fluorescent protein, StayGold, to visualize in-cell self-assembly. Fluorescence anisotropy measurements and fluorescence resonance energy transfer imaging, in addition to TEM observations of the cells and super-resolution microscopy, revealed that StayGold-conjugated β-Annulus peptides self-assembled into the StayGold-decorated artificial viral capsid in a cell. Using these techniques, we achieved the in-cell self-assembly of an artificial viral capsid. |
| 巻・号 | 13(6) |
| ページ | 1842-1850 |
| 公開日 | 2024-6-21 |
| DOI | 10.1021/acssynbio.4c00135 |
| PMID | 38729919 |
| MeSH | Capsid* / chemistry Capsid* / metabolism Capsid Proteins* / chemistry Capsid Proteins* / genetics Capsid Proteins* / metabolism Fluorescence Polarization Fluorescence Resonance Energy Transfer* Luminescent Proteins / chemistry Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Electron, Transmission Peptides* / chemistry Virus Assembly |
| IF | 4.411 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |