| RRC ID |
86178
|
| 著者 |
Issa H, Loubaki L, Al Amri A, Zibara K, Almutairi MH, Rouabhia M, Semlali A.
|
| タイトル |
Eugenol as a potential adjuvant therapy for gingival squamous cell carcinoma.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Adoption of plant-derived compounds for the management of oral cancer is encouraged by the scientific community due to emerging chemoresistance and conventional treatments adverse effects. Considering that very few studies investigated eugenol clinical relevance for gingival carcinoma, we ought to explore its selectivity and performance according to aggressiveness level. For this purpose, non-oncogenic human oral epithelial cells (GMSM-K) were used together with the Tongue (SCC-9) and Gingival (Ca9-22) squamous cell carcinoma lines to assess key tumorigenesis processes. Overall, eugenol inhibited cell proliferation and colony formation while inducing cytotoxicity in cancer cells as compared to normal counterparts. The recorded effect was greater in gingival carcinoma and appears to be mediated through apoptosis induction and promotion of p21/p27/cyclin D1 modulation and subsequent Ca9-22 cell cycle arrest at the G0/G1 phase, in a p53-independent manner. At these levels, distinct genetic profiles were uncovered for both cell lines by QPCR array. Moreover, it seems that our active component limited Ca9-22 and SCC-9 cell migration respectively through MMP1/3 downregulation and stimulation of inactive MMPs complex formation. Finally, Ca9-22 behaviour appears to be mainly modulated by the P38/STAT5/NFkB pathways. In summary, we can disclose that eugenol is cancer selective and that its mediated anti-cancer mechanisms vary according to the cell line with gingival squamous cell carcinoma being more sensitive to this phytotherapy agent.
|
| 巻・号 |
14(1)
|
| ページ |
10958
|
| 公開日 |
2024-5-13
|
| DOI |
10.1038/s41598-024-60754-8
|
| PII |
10.1038/s41598-024-60754-8
|
| PMID |
38740853
|
| PMC |
PMC11091204
|
| MeSH |
Apoptosis* / drug effects
Carcinoma, Squamous Cell* / drug therapy
Carcinoma, Squamous Cell* / metabolism
Carcinoma, Squamous Cell* / pathology
Cell Cycle Checkpoints / drug effects
Cell Line, Tumor
Cell Movement / drug effects
Cell Proliferation* / drug effects
Chemotherapy, Adjuvant / methods
Eugenol* / pharmacology
Eugenol* / therapeutic use
Gingival Neoplasms* / drug therapy
Gingival Neoplasms* / metabolism
Gingival Neoplasms* / pathology
Humans
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
Ca9-22(RCB1976) |
