論文 - 詳細
| RRC ID | 86502 |
|---|---|
| 著者 | Nouso H, Tazawa H, Tanimoto T, Tani M, Watanabe H, Oyama T, Noma K, Kagawa S, Kobayashi H, Noda T, Kuroda S, Fujiwara T. |
| タイトル | Near-infrared Photoimmunotherapy Targeting High-risk Human Neuroblastoma Cells Expressing GD2. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Neuroblastoma (NB) is a primary malignant tumor of the peripheral sympathetic nervous system in infancy. Despite advances in treatment, the prognosis remains poor for high-risk NB patients. Although immunotherapy using anti-GD2 antibodies is available for high-risk NB, the therapeutic efficacy is insufficient. Near-infrared photoimmunotherapy (NIR-PIT) is an antitumor strategy that induces tumor-specific cytotoxicity by combining an antibody-photoabsorber conjugate (APC) with NIR light irradiation. In this study, we investigated the therapeutic efficacy of GD2-targeted NIR-PIT against human NB cells. MATERIALS AND METHODS:GD2 expression was analyzed on the surface of high-risk human NB cells (CHP-134, LA-N-5, IMR-32) and non-high-risk human NB cells (SK-N-SH) by flow cytometry. The APC was synthesized by incubating anti-GD2 antibody and IR700. The cytotoxic effect of GD2-targeted NIR-PIT was evaluated using the XTT assay. The distribution of dead cells within tumor spheres was evaluated using a live/dead assay. The in vivo antitumor effect of GD2-targeted NIR-PIT was assessed using a subcutaneous human NB xenograft tumor model. RESULTS:GD2 protein was expressed on the surface of CHP-134, LA-N-5, and IMR-32 cells but not SK-N-SH cells. GD2-targeted NIR-PIT significantly suppressed the viability of GD2-positive NB cells but not GD2-negative NB cells, compared to the control and monotherapy groups. GD2-targeted NIR-PIT significantly reduced the volume of GD2-positive CHP-134 tumor spheres by inducing the accumulation of dead cells. Subcutaneous CHP-134 xenograft tumor models demonstrated that GD2-targeted NIR-PIT significantly inhibited tumor growth compared with the control and monotherapy groups. CONCLUSION:GD2-targeted NIR-PIT is a promising antitumor strategy for treating high-risk NB tumors expressing GD2. |
| 巻・号 | 46(1) |
| ページ | 25-38 |
| 公開日 | 2026-1-1 |
| DOI | 10.21873/anticanres.17921 |
| PII | 46/1/25 |
| PMID | 41469108 |
| MeSH | Animals Cell Line, Tumor Female Gangliosides* / immunology Gangliosides* / metabolism Humans Immunoconjugates / pharmacology Immunotherapy* / methods Infrared Rays* Mice Mice, Nude Neuroblastoma* / immunology Neuroblastoma* / metabolism Neuroblastoma* / pathology Neuroblastoma* / therapy Phototherapy* / methods Xenograft Model Antitumor Assays |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | SK-N-SH(RCB0426) CHP-134(RCB0487) LA-N-5(RCB0485) |