| RRC ID |
86559
|
| Author |
Noda T, Uriu R, Mashiko D, Shinohara H, Qu Y, Taira A, Matzuk RM, Tahala D, Nakano M, Araki K, Yu Z, Zhang Y, Matzuk MM, Ikawa M.
|
| Title |
GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding.
|
| Journal |
Nat Commun
|
| Abstract |
More than 20 genes expressed in the male reproductive tract have been identified as essential factors for sperm migration to and through the utero-tubal junction (UTJ), and they are divided into ADAM3-dependent and ADAM3-independent pathways. In parallel, sperm having UTJ migration defects also show impaired binding to the zona pellucida (ZP). Herein, we demonstrate that knockout of Galntl5, encoding a sperm surface protein, causes impaired sperm binding with the UTJ and ZP, and null males have severe infertility. GALNTL5 appreciably disappears in sperm lacking Adam3 or Lypd4, required for ADAM3-dependent and ADAM3-independent pathways, and GALNTL5 binds to N-acetylgalactosamine (GalNAc) distributed on the UTJ and ZP. Blockage of GalNAc decreases the number of sperm binding to the UTJ and ZP. Thus, we unveil that GALNTL5 is a responsible factor for UTJ migration and sperm-ZP binding, and that sperm bind to the UTJ and ZP through interaction of GALNTL5 and GalNAc.
|
| Volume |
16(1)
|
| Pages |
8264
|
| Published |
2025-9-17
|
| DOI |
10.1038/s41467-025-63805-4
|
| PII |
10.1038/s41467-025-63805-4
|
| PMID |
40962834
|
| PMC |
PMC12443989
|
| MeSH |
ADAM Proteins
Acetylgalactosamine* / metabolism
Animals
Fallopian Tubes* / metabolism
Female
Humans
Infertility, Male / genetics
Infertility, Male / metabolism
Lectins* / genetics
Lectins* / metabolism
Male
Membrane Glycoproteins
Mice
Mice, Knockout
N-Acetylgalactosaminyltransferases* / genetics
N-Acetylgalactosaminyltransferases* / metabolism
Polypeptide N-acetylgalactosaminyltransferase
Protein Binding
Sperm Motility
Sperm-Ovum Interactions* / physiology
Spermatozoa* / metabolism
Uterus* / metabolism
Zona Pellucida* / metabolism
|
| IF |
12.121
|
| Resource |
| DNA material |
pCAG-Galntl5-3xFLAG (RDB21094) |
