論文 - 詳細
| RRC ID | 86559 |
|---|---|
| 著者 | Noda T, Uriu R, Mashiko D, Shinohara H, Qu Y, Taira A, Matzuk RM, Tahala D, Nakano M, Araki K, Yu Z, Zhang Y, Matzuk MM, Ikawa M. |
| タイトル | GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding. |
| ジャーナル | Nat Commun |
| Abstract |
More than 20 genes expressed in the male reproductive tract have been identified as essential factors for sperm migration to and through the utero-tubal junction (UTJ), and they are divided into ADAM3-dependent and ADAM3-independent pathways. In parallel, sperm having UTJ migration defects also show impaired binding to the zona pellucida (ZP). Herein, we demonstrate that knockout of Galntl5, encoding a sperm surface protein, causes impaired sperm binding with the UTJ and ZP, and null males have severe infertility. GALNTL5 appreciably disappears in sperm lacking Adam3 or Lypd4, required for ADAM3-dependent and ADAM3-independent pathways, and GALNTL5 binds to N-acetylgalactosamine (GalNAc) distributed on the UTJ and ZP. Blockage of GalNAc decreases the number of sperm binding to the UTJ and ZP. Thus, we unveil that GALNTL5 is a responsible factor for UTJ migration and sperm-ZP binding, and that sperm bind to the UTJ and ZP through interaction of GALNTL5 and GalNAc. |
| 巻・号 | 16(1) |
| ページ | 8264 |
| 公開日 | 2025-9-17 |
| DOI | 10.1038/s41467-025-63805-4 |
| PII | 10.1038/s41467-025-63805-4 |
| PMID | 40962834 |
| PMC | PMC12443989 |
| MeSH | ADAM Proteins Acetylgalactosamine* / metabolism Animals Fallopian Tubes* / metabolism Female Humans Infertility, Male / genetics Infertility, Male / metabolism Lectins* / genetics Lectins* / metabolism Male Membrane Glycoproteins Mice Mice, Knockout N-Acetylgalactosaminyltransferases* / genetics N-Acetylgalactosaminyltransferases* / metabolism Polypeptide N-acetylgalactosaminyltransferase Protein Binding Sperm Motility Sperm-Ovum Interactions* / physiology Spermatozoa* / metabolism Uterus* / metabolism Zona Pellucida* / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 32 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-Galntl5-3xFLAG (RDB21094) |