Reference - Detail
| RRC ID | 86574 |
|---|---|
| Author | Xie K, Yano S, Wang J, Yamakoshi S, Ohta T, Uto T, Sakai M, He X, Yoshizaki K, Kubota T, Ohnishi K, Hara T. |
| Title | The Yeast-Fermented Garlic and a Balance of Spermine/Spermidine Activates Autophagy via EGR1 Transcriptional Factor. |
| Journal | Mol Nutr Food Res |
| Abstract |
Spermine (SPM) and spermidine (SPD) are polyamines found in all organisms, and their concentrations can be regulated by ingestion. We demonstrated that yeast-fermented garlic (YF) extract significantly increased autophag flux in OUMS-36T-1 and HeLa cells expressing the fluorescent probe (GFP-LC3-RFP-LC3ΔG). YF-induced increase of autophagy occurred independently of mTORC1 signaling, and RNA-sequencing analysis revealed that EGR1 was the most significantly altered gene in YF-treated OUMS-36T-1 cells. YF-treated EGR1-deficient HAP1 cells displayed reduced autophagic flux (p < 0.05). YF-induced increasing of autophagic flux occurred via a specific SPM/SPD ratio. HAP1 cells treated with equivalent amounts of SPD or SPM as that found in YF did not increase autophagic flux (p > 0.05); however, treatment with SPD and SPM in the same ratio as that found in YF increased autophagic flux (p < 0.05). This specific SPM/SPD ratio reduced MG132-induced proteostress via EGR1-dependent pathways (p < 0.05). Thus, the SPM/SPD balance may regulate autophagy via EGR1-dependent pathways, and controlling this balance may provide a strategy to maintain cellular homeostasis. |
| Volume | 69(5) |
| Pages | e202400606 |
| Published | 2025-3-1 |
| DOI | 10.1002/mnfr.202400606 |
| PMID | 39945057 |
| PMC | PMC11874185 |
| MeSH | Autophagy* / drug effects Cell Line Early Growth Response Protein 1* / genetics Early Growth Response Protein 1* / metabolism Early Growth Response Protein 1* / physiology Fermentation Garlic* / chemistry HeLa Cells Humans Plant Extracts / pharmacology Signal Transduction Spermidine* / metabolism Spermidine* / pharmacology Spermine* / metabolism Spermine* / pharmacology |
| IF | 5.309 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pMRX-IP-GFP-LC3-RFP-LC3 delta G (RDB14600) pMRX-IP-GFP-LC3-RFP (RDB14601) |