RRC ID 866
Author Kawamura N, Kurokawa K, Ito T, Hamamoto H, Koyama H, Kaito C, Sekimizu K.
Title Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation.
Journal Genes Cells
Abstract The role of transcription termination process for gene expression regulation is poorly understood. Either a multicopy supply of the rof gene or bicyclomycin, both of which inhibit the transcription termination Rho factor, suppressed the increased sensitivity to oxidative stress of the rifampicin-resistant rpoB mutation in Escherichia coli. Multi-copy supply of the rnk gene also suppressed oxidative stress sensitivity, coincident with the recovery of the reduced concentration of nucleoside triphosphates in the mutant cells, which is one of the factors that affects transcription termination efficiency in vitro. Thus, an appropriate, nonexcessive termination frequency at Rho-dependent transcription terminators might contribute to oxidative stress survival. Clinical application of oxidative stress against drug resistant bacteria is also discussed.
Volume 10(5)
Pages 477-87
Published 2005-5-1
DOI 10.1111/j.1365-2443.2005.00849.x
PMID 15836776
MeSH DNA-Directed RNA Polymerases / genetics DNA-Directed RNA Polymerases / metabolism* Drug Resistance, Bacterial Escherichia coli / genetics Escherichia coli / physiology Mutation Oxidative Stress / physiology* Rho Factor / metabolism* Rifampin Staphylococcus aureus / genetics Staphylococcus aureus / physiology Time Factors Transcription, Genetic / physiology*
IF 1.922
Times Cited 9
Prokaryotes E. coli ME8079(AB1157)