論文 - 詳細
| RRC ID | 86739 |
|---|---|
| 著者 | Ozawa M, Taguchi J, Mori H, Kikuchi M, Muratani M, Yamamoto T, Yamada Y, Yoshida N, Ikawa M. |
| タイトル | PTBP1 supports mouse spermatogenesis by facilitating cytoskeletal organization through the mTORC2-PKCα pathway in Sertoli cells. |
| ジャーナル | Commun Biol |
| Abstract |
Somatic Sertoli cells in the seminiferous tubule provide structural and paracrine support for spermatogenesis. Proper regulation of Sertoli cell cytoskeleton dynamics is essential for this function; however, the regulatory mechanisms remain unclear. Here, we found that polypyrimidine tract-binding protein 1 (PTBP1) maintained cytoskeletal integrity in Sertoli cells via mTORC2 signaling. Sertoli cell-specific Ptbp1 deletion in mice resulted in germ cell loss, blood-testis barrier disruption, and male infertility. Transcriptomic analysis of purified Sertoli cells revealed the dysregulation of cytoskeletal and adhesion-related genes. RNA immunoprecipitation sequencing demonstrated that PTBP1 bound to Rictor mRNA. PTBP1 loss reduced RICTOR protein expression and downstream PKCα activation, impairing the F-actin cytoskeleton. Cytoskeletal defects caused by PTBP1 deficiency were restored by constitutively active PKCα, confirming the functional relevance of the mTORC2-PKCα axis. These findings highlighted PTBP1's role as a post-transcriptional regulator of male fertility through the maintenance of Sertoli cell architecture and spermatogenesis. |
| 公開日 | 2026-1-29 |
| DOI | 10.1038/s42003-026-09636-5 |
| PII | 10.1038/s42003-026-09636-5 |
| PMID | 41612039 |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 4.8 |
| リソース情報 | |
| 遺伝子材料 | pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |