論文 - 詳細
| RRC ID | 86764 |
|---|---|
| 著者 | Pathak GP, Spiltoir JI, Höglund C, Polstein LR, Heine-Koskinen S, Gersbach CA, Rossi J, Tucker CL. |
| タイトル | Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Optogenetic tools allow regulation of cellular processes with light, which can be delivered with spatiotemporal resolution. In previous work, we used cryptochrome 2 (CRY2) and CIB1, Arabidopsis proteins that interact upon light illumination, to regulate transcription with light in yeast. While adopting this approach to regulate transcription in mammalian cells, we observed light-dependent redistribution and clearing of CRY2-tethered proteins within the nucleus. The nuclear clearing phenotype was dependent on the presence of a dimerization domain contained within the CRY2-fused transcriptional activators. We used this knowledge to develop two different approaches to regulate cellular protein levels with light: a system using CRY2 and CIB1 to induce protein expression with light through stimulation of transcription, and a system using CRY2 and a LOV-fused degron to simultaneously block transcription and deplete protein levels with light. These tools will allow precise, bi-directional control of gene expression in a variety of cells and model systems. |
| 巻・号 | 45(20) |
| ページ | e167 |
| 公開日 | 2017-11-16 |
| DOI | 10.1093/nar/gkx260 |
| PII | 3744534 |
| PMID | 28431041 |
| PMC | PMC5714224 |
| MeSH | Animals Animals, Genetically Modified Arabidopsis / genetics Arabidopsis Proteins / genetics* Basic Helix-Loop-Helix Proteins / genetics* Cell Line Cryptochromes / genetics* DNA-Binding Proteins / genetics Gene Expression Regulation* HEK293 Cells Humans Light Optogenetics / methods* Transcriptional Activation / genetics* Zebrafish / genetics |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | HGn39D |