| Abstract |
Nonhuman primates (NHPs) are invaluable for modeling human diseases because of their high physiological and genetic similarity to humans. Lentiviral vectors, which offer significant advantages in terms of gene delivery and stable in vivo expression, are widely used to generate transgenic NHP models. The protocol detailed herein provides a comprehensive method for preparing lentiviral vectors for the generation of transgenic cynomolgus monkeys. We describe the procedures for packaging lentiviral vectors, including the transfection and enrichment of lentiviral particles. To optimize lentiviral production and ensure high-titer viral preparations, we utilized Sendai virus hemagglutinin-neuraminidase glycoproteins to enhance infection efficiency. This protocol is designed to support the production of high-quality lentiviral vectors, which are essential for achieving robust, uniform transgene expression in transgenic cynomolgus monkeys to advance human disease modeling and therapeutic research.
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