Reference - Detail
| RRC ID | 86859 |
|---|---|
| Author | Jargalsaikhan BE, Muto M, Ema M. |
| Title | Optimized Lentiviral Vector Production Using Dual-Pseudotyping with VSV-G and Sendai Virus HN Glycoproteins for Enhanced Gene Delivery in Diverse Cell Types. |
| Journal | Methods Mol Biol |
| Abstract |
Highly efficient gene delivery using lentiviral vectors is beneficial not only for basic cell science research but also for human gene therapy applications. Lentiviral pseudotyping with vesicular stomatitis virus G (VSV-G) is a commonly used method that enables the transduction of various cell types. However, the low expression of low-density lipoprotein-receptor, the primary receptor of VSV-G, limits the efficiency of lentiviral vector transduction. Sendai virus hemagglutinin-neuraminidase glycoproteins recognize terminal sialic acids on the host cell plasma membrane, facilitating viral entry. Here, we describe methods for lentiviral dual-pseudotyping with VSV-G and Sendai virus hemagglutinin-neuraminidase to broaden viral tropism. |
| Volume | 2974 |
| Pages | 51-59 |
| Published | 2026-1-1 |
| DOI | 10.1007/978-1-0716-4807-0_5 |
| PMID | 41273640 |
| MeSH | Cell Line Gene Transfer Techniques* Genetic Vectors* / genetics HEK293 Cells HN Protein* / genetics HN Protein* / metabolism Humans Lentivirus* / genetics Membrane Glycoproteins Sendai virus* / genetics Transduction, Genetic / methods Viral Envelope Proteins* / genetics Viral Envelope Proteins* / metabolism Viral Tropism |
| Resource | |
| DNA material | CS-CA-GFP (RDB05964) pCAG-HIVgp (RDB04394) pRSV-Rev (RDB08121) pCMV-VSV-G (RDB04392) |