| RRC ID |
87230
|
| Author |
Nakatani S, Ueda H, Kawata A, Sato Y, Inomata K, Mano H, Wada M, Kobata K.
|
| Title |
Calcium-sensing receptor induces the apoptosis of chondrocytes in cooperation with phosphate transporter.
|
| Journal |
FEBS Open Bio
|
| Abstract |
Excess extracellular inorganic phosphate ions (Pi) and calcium ions (Ca2+) cause apoptosis and subsequent mineralization of chondrocytes. Here, we investigated the mechanism underlying the effect of these minerals. The chondrogenic cell line ATDC5 was treated with 2 mm Pi and/or Ca2+, and apoptosis, mineralization, and intracellular Pi concentrations were determined. Further, Pi- and Ca2+-treated cells were incubated with the Pi transporter (Pit-1) blocker phosphonoformic acid (PFA), the calcium-sensing receptor (CaSR) antagonist NPS-2143, and the CaSR agonist GdCl3. Individual addition of Pi and Ca2+ did not induce apoptosis and mineralization, while combined addition of the minerals induced both. The Pit-1 blocker and the CaSR antagonist completely inhibited the apoptosis induced by combined addition of Pi and Ca2+. Intracellular Pi concentration was remarkably increased by combined addition of Pi and Ca2+ as compared to the findings for individual addition. The Pit-1 blocker and CaSR antagonist completely inhibited the increase in intracellular Pi concentration induced by the combined addition of Pi and Ca2+. The CaSR agonist considerably increased the intracellular Pi concentration. Our results indicate that excess extracellular Ca2+ activates CaSR, which induces the intake of excess extracellular Pi through Pit-1 into ATDC5 cells. The resulting increase in intracellular Pi concentration induces apoptosis.
|
| Volume |
16(3)
|
| Pages |
496-502
|
| Published |
2026-3-1
|
| DOI |
10.1002/2211-5463.70142
|
| PMID |
41081312
|
| PMC |
PMC12955750
|
| MeSH |
Animals
Apoptosis* / drug effects
Apoptosis* / physiology
Calcium / metabolism
Cell Line
Chondrocytes* / cytology
Chondrocytes* / drug effects
Chondrocytes* / metabolism
Mice
Phosphate Transport Proteins* / metabolism
Phosphates / metabolism
Receptors, Calcium-Sensing* / agonists
Receptors, Calcium-Sensing* / antagonists & inhibitors
Receptors, Calcium-Sensing* / metabolism
|
| Resource |
| Human and Animal Cells |
ATDC5(RCB0565) |
