Reference - Detail
| RRC ID | 87230 |
|---|---|
| Author | Nakatani S, Ueda H, Kawata A, Sato Y, Inomata K, Mano H, Wada M, Kobata K. |
| Title | Calcium-sensing receptor induces the apoptosis of chondrocytes in cooperation with phosphate transporter. |
| Journal | FEBS Open Bio |
| Abstract |
Excess extracellular inorganic phosphate ions (Pi) and calcium ions (Ca2+) cause apoptosis and subsequent mineralization of chondrocytes. Here, we investigated the mechanism underlying the effect of these minerals. The chondrogenic cell line ATDC5 was treated with 2 mm Pi and/or Ca2+, and apoptosis, mineralization, and intracellular Pi concentrations were determined. Further, Pi- and Ca2+-treated cells were incubated with the Pi transporter (Pit-1) blocker phosphonoformic acid (PFA), the calcium-sensing receptor (CaSR) antagonist NPS-2143, and the CaSR agonist GdCl3. Individual addition of Pi and Ca2+ did not induce apoptosis and mineralization, while combined addition of the minerals induced both. The Pit-1 blocker and the CaSR antagonist completely inhibited the apoptosis induced by combined addition of Pi and Ca2+. Intracellular Pi concentration was remarkably increased by combined addition of Pi and Ca2+ as compared to the findings for individual addition. The Pit-1 blocker and CaSR antagonist completely inhibited the increase in intracellular Pi concentration induced by the combined addition of Pi and Ca2+. The CaSR agonist considerably increased the intracellular Pi concentration. Our results indicate that excess extracellular Ca2+ activates CaSR, which induces the intake of excess extracellular Pi through Pit-1 into ATDC5 cells. The resulting increase in intracellular Pi concentration induces apoptosis. |
| Volume | 16(3) |
| Pages | 496-502 |
| Published | 2026-3-1 |
| DOI | 10.1002/2211-5463.70142 |
| PMID | 41081312 |
| PMC | PMC12955750 |
| MeSH | Animals Apoptosis* / drug effects Apoptosis* / physiology Calcium / metabolism Cell Line Chondrocytes* / cytology Chondrocytes* / drug effects Chondrocytes* / metabolism Mice Phosphate Transport Proteins* / metabolism Phosphates / metabolism Receptors, Calcium-Sensing* / agonists Receptors, Calcium-Sensing* / antagonists & inhibitors Receptors, Calcium-Sensing* / metabolism |
| IF | 2.231 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) |