論文 - 詳細
| RRC ID | 87263 |
|---|---|
| 著者 | Tsuruta-Kishino T, Koya J, Kataoka K, Narukawa K, Sumitomo Y, Kobayashi H, Sato T, Kurokawa M. |
| タイトル | Loss of p53 induces leukemic transformation in a murine model of Jak2 V617F-driven polycythemia vera. |
| ジャーナル | Oncogene |
| Abstract |
As leukemic transformation of myeloproliferative neoplasms (MPNs) worsens the clinical outcome, reducing the inherent risk of the critical event in MPN cases could be beneficial. Among genetic alterations concerning the transformation, the frequent one is TP53 mutation. Here we show that retroviral overexpression of Jak2 V617F mutant into wild-type p53 murine bone marrow cells induced polycythemia vera (PV) in the recipient mice, whereas Jak2 V617F-transduced p53-null mice developed lethal leukemia after the preceding PV phase. The leukemic mice had severe anemia, hepatosplenomegaly, pulmonary hemorrhage and expansion of dysplastic erythroid progenitors. Primitive leukemia cells (c-kit+Sca1+Lin- (KSL) and CD34-CD16/32-c-kit+Sca1-Lin- (megakaryocyte-erythroid progenitor; MEP)) and erythroid progenitors (CD71+) from Jak2 V617F-transduced p53-null leukemic mice had leukemia-initiating capacity, however, myeloid differentiated populations (Mac-1+) could not recapitulate the disease. Interestingly, recipients transplanted with CD71+ cells rapidly developed erythroid leukemia, which was in sharp contrast to leukemic KSL cells to cause lethal leukemia after the polycythemic state. The leukemic CD71+ cells were more sensitive to INCB18424, a potent JAK inhibitor, than KSL cells. p53 restoration could ameliorate Jak2 V617F-transduced p53-null erythroleukemia. Taken together, our results show that p53 loss is sufficient for inducing leukemic transformation in Jak2 V617F-positive MPN. |
| 巻・号 | 36(23) |
| ページ | 3300-3311 |
| 公開日 | 2017-6-8 |
| DOI | 10.1038/onc.2016.478 |
| PII | onc2016478 |
| PMID | 28068330 |
| MeSH | Animals Apoptosis Bone Marrow / metabolism Bone Marrow / pathology Cell Proliferation Cell Transformation, Neoplastic / genetics Cell Transformation, Neoplastic / metabolism Cell Transformation, Neoplastic / pathology* Female Janus Kinase 2 / genetics* Leukemia, Experimental / genetics Leukemia, Experimental / metabolism Leukemia, Experimental / pathology* Male Mice Mice, Inbred C57BL Mice, Knockout Mutation / genetics* Polycythemia Vera / genetics Polycythemia Vera / metabolism Polycythemia Vera / pathology* Signal Transduction Tumor Cells, Cultured Tumor Suppressor Protein p53 / physiology* |
| IF | 7.971 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01361 |