| RRC ID |
87278
|
| Author |
Cui L, Sun W, Yu M, Li N, Guo L, Gu H, Zhou Y.
|
| Title |
Disrupted-in-schizophrenia1 (DISC1) L100P mutation alters synaptic transmission and plasticity in the hippocampus and causes recognition memory deficits.
|
| Journal |
Mol Brain
|
| Abstract |
Disrupted-in-schizophrenia 1(DISC1) is a promising candidate susceptibility gene for a spectrum of psychiatric illnesses that share cognitive impairments in common, including schizophrenia, bipolar disorder and major depression. Here we report that DISC1 L100P homozygous mutant shows normal anxiety- and depression-like behavior, but impaired object recognition which is prevented by administration of atypical antipsychotic drug clozapine. Ca2+ image analysis reveals suppression of glutamate-evoked elevation of cytoplasmic [Ca2+] in L100P hippocampal slices. L100P mutant slices exhibit decreased excitatory synaptic transmission (sEPSCs and mEPSCs) in dentate gyrus (DG) and impaired long-term potentiation in the CA1 region of the hippocampus. L100P mutation does not alter proteins expression of the excitatory synaptic markers, PSD95 and synapsin-1; neither does it changes dendrites morphology of primary cultured hippocampal neurons. Our findings suggest that the existence of abnormal synaptic transmission and plasticity in hippocampal network may disrupt declarative information processing and contribute to recognition deficits in DISC1 L100P mutant mice.
|
| Volume |
9(1)
|
| Pages |
89
|
| Published |
2016-10-12
|
| DOI |
10.1186/s13041-016-0270-y
|
| PII |
10.1186/s13041-016-0270-y
|
| PMID |
27729083
|
| PMC |
PMC5059944
|
| MeSH |
Animals
Anxiety / complications
Anxiety / drug therapy
Anxiety / physiopathology
Behavior, Animal
Calcium / metabolism
Clozapine / pharmacology
Clozapine / therapeutic use
Dendrites / drug effects
Dendrites / metabolism
Dendrites / pathology
Depression / complications
Depression / drug therapy
Depression / physiopathology
Female
Hippocampus / drug effects
Hippocampus / physiopathology*
Homozygote
Male
Memory Disorders / drug therapy
Memory Disorders / genetics*
Memory Disorders / physiopathology*
Mice, Inbred C57BL
Motor Activity / drug effects
Mutation / genetics*
Nerve Tissue Proteins / genetics*
Neuronal Plasticity / drug effects
Neuronal Plasticity / genetics*
Recognition, Psychology* / drug effects
Spatial Learning / drug effects
Synaptic Transmission / drug effects
Synaptic Transmission / genetics*
|
| IF |
4.686
|
| Resource |
| Mice |
RBRC02324 |
