論文 - 詳細
| RRC ID | 87312 |
|---|---|
| 著者 | Nagata W, Kodama K, Nakagawa K, Ishizuka T. |
| タイトル | Lysophosphatidic acid regulates macrophage polarization via LPAR1 by suppressing inflammatory responses and promoting M2-like characteristics. |
| ジャーナル | Clin Exp Immunol |
| Abstract |
Lysophosphatidic acid (LPA) is a crucial bioactive lipid mediator involved in various physiological processes; however, its role in macrophage polarization remains poorly understood; therefore, this study aimed to elucidate the modulatory effect of LPA on macrophage polarization, particularly its ability to shift M1 macrophages towards an M2-like phenotype, using murine macrophage RAW264.7 cells to confirm the expression of LPA receptor 1 (LPAR1) through immunofluorescence staining, which revealed that treatment of resting MO macrophages with LPA decreased inflammatory cytokines (IL-6, TNF-α) and increased TGF-β, with similar effects observed in LPS-stimulated cells and reversed by the LPAR1 inhibitor AM095, and immunostaining demonstrated a notable shift from an M1- to M2-like phenotype, as evidenced by an increase in the arginase-1/CD68 ratio; furthermore, LPA significantly decreased lactate production and increased ATP production in M1 macrophages, promoting a shift towards oxidative phosphorylation and suggesting metabolic reprogramming towards an M2-like phenotype, significantly influencing macrophage polarization and promoting a shift from a pro-inflammatory M1-like phenotype to an anti-inflammatory M2-like phenotype; these results suggest that treatment with LPA may help ameliorate diseases characterized by aberrant macrophage polarization, providing insights for the development of potential therapeutic strategies for inflammatory and autoimmune diseases. |
| 巻・号 | 220(1) |
| 公開日 | 2026-1-6 |
| DOI | 10.1093/cei/uxaf083 |
| PII | 8385282 |
| PMID | 41423802 |
| PMC | PMC12782111 |
| MeSH | Animals Cell Polarity / drug effects Cytokines / metabolism Inflammation* / immunology Inflammation* / metabolism Lipopolysaccharides / pharmacology Lysophospholipids* / pharmacology Macrophage Activation / drug effects Macrophages* / drug effects Macrophages* / immunology Macrophages* / metabolism Mice RAW 264.7 Cells Receptors, Lysophosphatidic Acid* / metabolism |
| IF | 3.532 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.7 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB053) |