Reference - Detail
| RRC ID | 87361 |
|---|---|
| Author | Dev K, Yurtsever I, Bhadra S, Guduri YA, Davi K, Xu YJ. |
| Title | Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays. |
| Journal | Methods Mol Biol |
| Abstract |
Hydroxyurea is an inhibitor of ribonucleotide reductase and is commonly used in laboratories to induce replication stress or arrest cells in the S phase for cell cycle or checkpoint studies. However, hydroxyurea also causes side effects such as oxidative stress, particularly under chronic exposure conditions. This complicates the interpretation of the cell-killing mechanisms, particularly in a previously uncharacterized mutant, and thus hampers the analyses. Here, we describe a few easy and simple spot assays in fission yeast that allow dissecting the cell-killing mechanisms of hydroxyurea. |
| Volume | 2862 |
| Pages | 267-276 |
| Published | 2025-1-1 |
| DOI | 10.1007/978-1-0716-4168-2_19 |
| PMID | 39527207 |
| PMC | PMC11667678 |
| MeSH | Hydroxyurea* / pharmacology Oxidative Stress / drug effects Schizosaccharomyces* / drug effects |
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| Total number of mentions | 0 |
| Resource | |
| Yeast | |