| RRC ID |
87361
|
| Author |
Dev K, Yurtsever I, Bhadra S, Guduri YA, Davi K, Xu YJ.
|
| Title |
Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays.
|
| Journal |
Methods Mol Biol
|
| Abstract |
Hydroxyurea is an inhibitor of ribonucleotide reductase and is commonly used in laboratories to induce replication stress or arrest cells in the S phase for cell cycle or checkpoint studies. However, hydroxyurea also causes side effects such as oxidative stress, particularly under chronic exposure conditions. This complicates the interpretation of the cell-killing mechanisms, particularly in a previously uncharacterized mutant, and thus hampers the analyses. Here, we describe a few easy and simple spot assays in fission yeast that allow dissecting the cell-killing mechanisms of hydroxyurea.
|
| Volume |
2862
|
| Pages |
267-276
|
| Published |
2025-1-1
|
| DOI |
10.1007/978-1-0716-4168-2_19
|
| PMID |
39527207
|
| PMC |
PMC11667678
|
| MeSH |
Hydroxyurea* / pharmacology
Oxidative Stress / drug effects
Schizosaccharomyces* / drug effects
|
| Resource |
| Yeast |
|
