| RRC ID |
87361
|
| 著者 |
Dev K, Yurtsever I, Bhadra S, Guduri YA, Davi K, Xu YJ.
|
| タイトル |
Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays.
|
| ジャーナル |
Methods Mol Biol
|
| Abstract |
Hydroxyurea is an inhibitor of ribonucleotide reductase and is commonly used in laboratories to induce replication stress or arrest cells in the S phase for cell cycle or checkpoint studies. However, hydroxyurea also causes side effects such as oxidative stress, particularly under chronic exposure conditions. This complicates the interpretation of the cell-killing mechanisms, particularly in a previously uncharacterized mutant, and thus hampers the analyses. Here, we describe a few easy and simple spot assays in fission yeast that allow dissecting the cell-killing mechanisms of hydroxyurea.
|
| 巻・号 |
2862
|
| ページ |
267-276
|
| 公開日 |
2025-1-1
|
| DOI |
10.1007/978-1-0716-4168-2_19
|
| PMID |
39527207
|
| PMC |
PMC11667678
|
| MeSH |
Hydroxyurea* / pharmacology
Oxidative Stress / drug effects
Schizosaccharomyces* / drug effects
|
| リソース情報 |
| 酵母 |
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