Reference - Detail
| RRC ID | 87545 |
|---|---|
| Author | Knudsen C, Moriya A, Nakato E, Gulati R, Akiyama T, Nakato H. |
| Title | Chondroitin sulfate regulates proliferation of Drosophila intestinal stem cells. |
| Journal | PLoS Genet |
| Abstract |
The basement membrane (BM) plays critical roles in stem cell maintenance and activity control. Here we show that chondroitin sulfate (CS), a major component of the Drosophila midgut BM, is required for proper control of intestinal stem cells (ISCs). Loss of Chsy, a critical CS biosynthetic gene, resulted in elevated levels of ISC proliferation during homeostasis, leading to midgut hyperplasia. Regeneration assays demonstrated that Chsy mutant ISCs failed to properly downregulate mitotic activity at the end of regeneration. We also found that CS is essential for the barrier integrity to prevent leakage of the midgut epithelium. CS is known to be polymerized by the action of the complex of Chsy and another critical protein, Chondroitin polymerizing factor (Chpf). We found that Chpf mutants show increased ISC division during midgut homeostasis and regeneration, similar to Chsy mutants. As Chpf is induced by a tissue damage during regeneration, our data suggest that Chpf functions with Chsy to facilitate CS remodeling and stimulate tissue repair. We propose that the completion of the repair of CS-containing BM acts as a prerequisite to properly terminate the regeneration process. |
| Volume | 21(5) |
| Pages | e1011686 |
| Published | 2025-5-1 |
| DOI | 10.1371/journal.pgen.1011686 |
| PII | PGENETICS-D-24-00679 |
| PMID | 40343906 |
| PMC | PMC12063844 |
| MeSH | Animals Basement Membrane / metabolism Cell Proliferation Chondroitin Sulfates* / genetics Chondroitin Sulfates* / metabolism Drosophila / genetics Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics Homeostasis Intestinal Mucosa / metabolism Intestines / cytology Intestines / growth & development Mutation Regeneration / genetics Stem Cells* / cytology Stem Cells* / metabolism |
| IF | 5.175 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.5 |
| Resource | |
| Drosophila | DGRC#110807 DGRC#112001 DGRC#113886 |