Reference - Detail
| RRC ID | 87656 |
|---|---|
| Author | Almasoud J, Andrieu C, Lee BH, Franz A. |
| Title | The adipose tissue has an apical-basal polarity required for Col IV-dependent cell-cell adhesion. |
| Journal | J Cell Biol |
| Abstract |
In epithelia, the apical-basal polarity machinery positions E-cadherin-based adherens junctions at the apical-lateral border to mediate cell-cell adhesion. The Drosophila adipose tissue, the fat body, forms a monolayer in which integrin-binding to collagen IV intercellular concentrations mediates cell-cell adhesion. How these atypical adhesion complexes form is unknown. Here we show that the fat body has apical-basal polarity, with aPKC, Crumbs, and Par-6 on the opposite side of Lgl and Dlg. Collagen IV, Laminin, Perlecan, and Nidogen are abundant in the basal basement membrane, while collagen IV predominates in the apical basement membrane. Crumbs, aPKC, Scribble, and Lgl knockdown in the fat body lead to cell-cell adhesion defects. Moreover, aPKC is essential for the formation of collagen IV intercellular concentrations. We further show that during fat body remodeling, Ecdysone regulates the loss of apical-basal polarity and collagen IV intercellular concentrations to induce cell-cell dissociation and swimming migration. Our work hence uncovers a novel role for apical-basal polarity in the Drosophila adipose tissue in regulating cell-cell adhesion via collagen IV intercellular concentrations. |
| Volume | 225(3) |
| Published | 2026-3-2 |
| DOI | 10.1083/jcb.202504139 |
| PII | 281356 |
| PMID | 41493271 |
| PMC | PMC12772501 |
| MeSH | Adipose Tissue* / cytology Adipose Tissue* / metabolism Animals Basement Membrane / metabolism Cell Adhesion / physiology Cell Movement Cell Polarity* / physiology Collagen Type IV* / genetics Collagen Type IV* / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster* / cytology Drosophila melanogaster* / metabolism Fat Body* / cytology Fat Body* / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Protein Kinase C / genetics Protein Kinase C / metabolism Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism |
| IF | 8.811 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 33 |
| Altmetric score changes over past 6months | 9.1 |
| Resource | |
| Drosophila | DGRC#110836 DGRC#110626 DGRC#115467 |