論文 - 詳細
| RRC ID | 87686 |
|---|---|
| 著者 | Kawasaki T, Nishimura T, Tani N, Ramos C, Karaulanov E, Shinya M, Saito K, Taylor E, Ketting RF, Ishiguro KI, Tanaka M, Siegfried KR, Sakai N. |
| タイトル | Meioc-Piwil1 complexes regulate rRNA transcription for differentiation of spermatogonial stem cells. |
| ジャーナル | Elife |
| Abstract |
Ribosome biogenesis is vital for sustaining stem cell properties, yet its regulatory mechanisms are obscure. Herein, we show unique properties of zebrafish meioc mutants in which spermatogonial stem cells (SSCs) do not differentiate or upregulate rRNAs. Meioc colocalized with Piwil1 in perinuclear germ granules, but Meioc depletion resulted in Piwil1 accumulation in nucleoli. Nucleolar Piwil1 interacted with 45S pre-rRNA. piwil1+/- spermatogonia with reduced Piwil1 upregulated rRNAs, and piwil1+/-;meioc-/- spermatogonia recovered differentiation later than those in meioc-/-. Further, Piwil1 interacted with Setdb1 and HP1α, and meioc-/- spermatogonia exhibited high levels of H3K9me3 and methylated CpG in the 45S-rDNA region. These results indicate that zebrafish SSCs maintain low levels of rRNA transcription with repressive marks similar to Drosophila piRNA targets of RNA polymerase II, and that Meioc has a unique function on preventing localization of Piwil1 in nucleoli to upregulate rRNA transcripts and to promote SSC differentiation. |
| 巻・号 | 14 |
| 公開日 | 2025-7-24 |
| DOI | 10.7554/eLife.104295 |
| PII | 104295 |
| PMID | 40705004 |
| PMC | PMC12289311 |
| MeSH | Adult Germline Stem Cells* / physiology Animals Argonaute Proteins* / genetics Argonaute Proteins* / metabolism Cell Differentiation* Male RNA, Ribosomal* / biosynthesis RNA, Ribosomal* / genetics RNA, Ribosomal* / metabolism Spermatogonia* / cytology Spermatogonia* / metabolism Spermatogonia* / physiology Transcription, Genetic* Zebrafish Zebrafish Proteins* / genetics Zebrafish Proteins* / metabolism |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(sox17:EGFP) |